响应面法优化卑霉素发酵培养基的研究  

Response Surface Methdology for Optimizing Avilamycin Fermentation Medium

作　　者：靳亮[1] 王泽立[1] 童应凯[2] 杨永涛[2] 祝静静[1] 孙翠霞[1] 

机构地区：[1]山东农业大学,泰安271018 [2]天津农学院,天津300384

出　　处：《生物技术通报》2007年第2期155-158,162,共5页Biotechnology Bulletin

摘　　要：在对卑霉素发酵培养基进行全因子试验和最陡爬坡试验后,运用Box-Behken设计的响应面试验(Response-surface experiment)对绿色产色链霉菌TN-29进行卑霉素发酵培养基的优化。响应面法对40h分批发酵结果的优化分析表明:豆饼粉、甘露醇及葡萄糖三种因素对卑霉素无明显的交互作用,在豆饼粉、甘露醇和葡萄糖的浓度分别为3.61%,2.99%,0.58%时,发酵液中效价值达到77.9U/ml的最大值。After we conducted the fermentation of TN-29 with full factorial experiment and the steepest ascent search experiment,rsponse surface experiments with the Box-Behnken design were conducted on TN-29 to determine the optimal medium for avilamycin fermentation.By using response surface analysis,the results of 40 hours avilamycin fermentation showed that there is no any cross-effection on avilamycin fermentation among soybean cake mannose and glucose,and the maximal titer （77.9U/ml）appeared at the region where respective concentration of soybean cake power, mannose and glucose were around 3.61%,2.99%,0.58%.

关 键 词：响应面法 优化 卑霉素 发酵培养基 

分 类 号：Q93-33[生物学—微生物学]