一株高效产氢突变体RF-9的筛选与产氢特性  

Screening and H_2-producing characters of a high efficient H_2-producing mutant RF-9

作　　者：郑国香[1] 任南琪[1] 李永峰[1] 林海龙[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090

出　　处：《中国环境科学》2007年第2期184-188,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(30470054);国家"973"项目(G2000026402)

摘　　要：以从连续流搅拌槽式反应器(CSTR)内的活性污泥中分离出的厌氧产氢菌Ethanologenens sp.ZGX4为出发菌株,经过紫外线和亚硝酸复合诱变,从大量的突变体中筛选出一株稳定的高效产氢菌株RF-9.磷酸盐浓度在120mmol/L、温度37℃、初始pH6.0、葡萄糖浓度10g/L培养条件下,RF-9的单位体积产氢量和氢气产率为139.7mmol/L和2.52mol H2/mol葡萄糖,分别是对照的1.50倍和1.43倍.在发酵时间为15h、pH4.1、细胞干重0.722g/L时,RF-9获得其最大产氢速率345mLH2/(h·L),是对照ZGX4的1.41倍.RF-9的主要液相末端产物为乙醇和乙酸,为典型的乙醇发酵细菌,与对照相似.Ethanoligenens sp. ZGX4 the anaerobic hydrogen producing （HP） bacterium isolated from a continuous stirred tank reactor （CSTR） was used as wild parent strain, through UV and nitrous acid combined mutation, a stable highly effective HP strain RF-9 was screened from large amount of mutant. At cultural condition of phosphate of 120mmol/L, temperature of 37℃, original pH of 6.0 and glucose concentration of 10g/L, unit volume HP amount and hydrogen yield were 139.7mmol/L and 2.52mol HE/mOl glucose, being 1.50 and 1.43 times of the contrast respectively. At fermentation time of 15h, pH 4.1 and cell dry weight of 0.722g/L, RF-9 obtained its maximal HP rate of 345mL HE/（h·L） being its contrast 1.41times. The main liquid end products of RF-9 were ethanol and acetic acids, being the typical ethanol fermentation bacteria, similar to the contrasts.

关 键 词：生物制氢 复合诱变 突变体 产氢性能 

分 类 号：X172[环境科学与工程—环境科学]