猪生长激素基因与白介素-6基因在小鼠体内的表达效应分析  

Expression effect analysis of the mice inject with the plasmids for porcine growth hormone gene and porcine interlukin-6 gene in vivo

作　　者：谭俊杰[1] 杨涛[1] 徐敏[1] 刘润叶[1] 刘世贵[1] 龙章富[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2007年第2期447-450,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：教育部重点项目(国教00104);四川省应用基础项目(02NY029-079)

摘　　要：比较研究了阳离子脂质体包裹VpGH、VpGH联合VpIL-6以及脂质体(对照)腹腔注射昆明小鼠的增长效应与免疫应答.结果表明,VpGH注射小鼠、VpGH联合VpIL-6注射小鼠在体重增长幅度和血清GH水平上均较对照组显著增高,在注射后第4周效果最明显;VpGH联合VpIL-6注射小鼠较仅注射VpGH小鼠在增重幅度和血清GH水平显著增高的趋势明显.但在白细胞数量、红细胞数量上较对照组除第1,2两周外,其余时间段无显著差异.在IgG表达上各组均无显著差异.The growth effects and immunity responses of Kunming mice were compared with three disposals： celiac injection with cation-lipid-wrapped VpGH, VpGH plus VpIL-6 and pure lipid as control. The result revealed that indices of the rats weight gain rate and plasina GH by VpGH solely and co-expression of VPGH and VpIL-6 were evidently boosted up compared with those of rats disposed by control, and it was the biggest effect in the 4th week. Co-expression of VPGH and VpIL-6 were evidently boosted up compared with the ones of rats disposed by VpGH genes solely. Besides 1st and 2nd weeks, the number of white blood cell and red blood cell does not show remarkable difference with the control. In the contents of IgG, three disposals do not show remarkable difference in all time,

关 键 词：猪 GH IL-6 基因表达 小鼠 

分 类 号：Q78[生物学—分子生物学]