两份转Fer基因富铁水稻特异种质应用价值的初步评价  

Evaluation and Application of Two High-Iron Transgenic Rice Lines with a Pea Ferritin Gene

作　　者：叶红霞[1] 郭泽建[2] 舒庆尧[1] 徐晓晖[3] 包劲松[1] 沈圣泉[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310029 [2]中国农业大学植物病理系,北京100094 [3]中国计量学院生命科学学院,浙江杭州310018

出　　处：《中国水稻科学》2007年第3期270-274,共5页Chinese Journal of Rice Science

基　　金：浙江省"0406"计划重大专项资助项目(2004C12020-1);浙江省科技攻关资助项目(2006C32015)

摘　　要：在获得了105份转豌豆铁蛋白基因(Fer)水稻转化体的基础上,通过连续7代自交纯化,并结合GUS标记辅助选择,得到了82份独立转基因纯系。经稻米矿质元素检测,发掘到2份富铁转基因种质(Fer34和Fer65),其精米全铁含量为野生型亲本秀水11的4.82倍和3.46倍。进一步鉴定表明,所筛选到的2份富铁转基因种质的外源基因(Fer)遗传简单,增加精米粉铁含量明显,对植株重要农艺性状、其他矿质营养、精米外观和食用品质等均无明显的负作用,是较为难得的富铁转基因优良新种质。此外,还就该2份富铁种质的实用价值及其应用前景进行了讨论。A total of 105 transgenic rice lines independently transformed with a pea ferritin gene （Fer） were previously obtained. After seven generations of self-crossing and GUS marker assisted selection, 82 homozygous transgenic rice lines were used to evaluate their potential applications as high-iron rice germplasm. Two high-iron transgenic rice lines Fer34 and Fer65, with the iron content in the milled rice 4.82 and 3.46 times of the wildparent Xiushui 11 were identified. Further analysis indicated that the exogenous Fer gene was highly expressed, and inherited as a single locus. The transgene had no negative effect on the agronomic traits of rice plants, other mineral nutritional components, appearance and eating quality of the milled rice, indicating that they were elite high-iron breeding lines. Furthermore, the practical application and further studies facilitating utilization of the two elite breeding lines were discussed.

关 键 词：转基因水稻 豌豆铁蛋白基因 矿质营养 稻米品质 

分 类 号：S511[农业科学—作物学]