姜黄素对人卵巢癌细胞株HO-8910增殖和凋亡的影响  

Effects of Curcumin on Proliferation and Apoptosis of Human Ovarian Cancer Cell Line HO-8910 in vitro

作　　者：刘海艳[1] 温玉库[1] 

机构地区：[1]辽宁医学院附属第一医院妇产科,辽宁省锦州市121000

出　　处：《医学理论与实践》2007年第5期501-503,共3页The Journal of Medical Theory and Practice

摘　　要：目的：探讨姜黄素对体外培养人卵巢癌HO-8910细胞增殖和凋亡的影响。方法：选用人卵巢癌细胞株HO-8910进行体外培养至对数生长期,以0-80μmol/L姜黄素（5μmol/L,10μmol/L,20μmol/L、40μmol/L、80μmol/L）分别处理HO-8910细胞24、48、72h,四甲基偶氮唑蓝（MTT）实验测定细胞的生长抑制率;流式细胞仪检测不同浓度药物对细胞凋亡率的影响;免疫组化法检测PCNA在细胞中的表达,评价细胞的增殖状态。结果：姜黄素对HO-8910细胞增殖抑制率随药物浓度增加及作用时间延长有升高趋势,呈时间剂量依赖性。姜黄素作用24h随浓度的升高PCNA阳性表达逐渐降低。流式细胞仪分析证实随着浓度的升高细胞凋亡率和膜受损率均提高。结论：姜黄素对人卵巢癌细胞株HO-8910的生长有显著的抑制作用,并能诱导其凋亡。Objective： To investigate the effects of curcumin on proliferation and apoptosis of human ovarian cancer cell line HO-8910 in vitro. Methods： The human ovarian cancer cell line HO-8910 was cultured to logarithm growing period and treated with curcumin of different concentration（5μmol/L, 10μmol/L,20μmol/L,40μmol/L,80μmol/L）. The inhibition rates of ceils were detected through MTT assay; the apoptosis rates of cells was tested by flow cytometry; the expression of PCNA in HO-8910 cell was observed by SABC imunohistochemistry. Results. Curcumin inhibited the proliferation of HO-8910 calls in a dose-and time-dependent manner. The tumor cell apopotic rate in curcumin group was significantly higher than those in control group. The expression of PCNA was increased in a dose dependent manner. Conclusion：The curcunm not only inhibits proliferation, but also induces apoptosis of human ovarian cancer cell line HO-8910.

关 键 词：姜黄素 卵巢癌 凋亡 

分 类 号：R73-75[医药卫生—肿瘤] R737.31[医药卫生—临床医学]