三氯化镧对CBRH-7919细胞Cyclin D_1基因表达的影响  

Effect of LaCl_3 on the expression of Cyclin D_1 in CBRH-7919 cells

作　　者：姜文华[1] 陈东[2] 郝利铭[1] 孟晓婷[1] 

机构地区：[1]吉林大学基础医学院组胚教研室,长春130021 [2]广东医学院组胚教研室

出　　处：《陕西医学杂志》2007年第5期524-526,共3页Shaanxi Medical Journal

基　　金：吉林省科技厅自然科学基金资助项目(No20030542-1)

摘　　要：目的:研究三氯化镧对大鼠肝癌细胞CyclinD1基因表达的影响,探讨三氯化镧抑制肝癌细胞生长的机制。方法:以体外培养大鼠肝癌细胞株CBRH-7919为研究对象,给予0.01、0.1、1.0mmol/LLaCl3,培养3、5、7d后,运用流式细胞术检测CBRH-7919的细胞周期时相变化。采用半定量逆转录-聚合酶链反应(RT-PCR)、免疫细胞化学技术检测CyclinD1的基因表达。结果:0.1、1.0mmol/LLaCl3组培养3、5、7d后,G0/G1期细胞百分数均有显著性增加;CyclinD1的转录和蛋白表达均显著减弱。结论:LaCl3可通过下调CyclinD1的转录和蛋白表达,使肿瘤细胞从G1期进入S期受阻,从而抑制CBRH-7919细胞的生长。Objective： To study the effect of LaCI3 on the expression of Cyclin D1 gene in CBRH-7919 cells, Methods ： The CBRH-7919 cells were treated with 0.01, 0.1, 1.0 mmol/L LaCl3 for 3,5,7 days in vitro respectively,and the changes of cell cycle were detected by flow cytometry, At the same time, The expression of Cyclin D1 gene was detected by RT-PCR arid immunocytochemisty. Results ： As compared with control group, The percentage of CBRH-7919 cells of G0/G1 was evidently increased in 0.1 and 1.0 mmol/L LaCl3 groups , while the expression of Cyclin D1 gene was decreased in 0. 1 and 1. 0mmol/L LaCl3 groups . Conclusion： LaCl3 can obviously inhibit the growth of CBRH-7919 cells by downregulating the expression of Cyclin D1 gene that blocks the cell cycle progress from G1to S.

关 键 词：肝肿瘤/病理生理学 细胞周期 基因/代谢 稀土元素/治疗作用 动物 实验 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]