山茱萸炮制前后宏微量元素及氨基酸成分比较研究  

Comparative Study on Contents of Amino Acid and Major and Trace Elements in Cornus officinalis before and after being Processed

作　　者：丁霞[1] 朱方石 余宗亮[3] 董李娜[3] 蔡宝昌[3] 

机构地区：[1]南京农业大学应化系,江苏南京210095 [2]江苏省中医药研究院,江苏南京210028 [3]南京中医药大学,江苏南京210029

出　　处：《中药材》2007年第4期396-399,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究山茱萸中宏微量元素和氨基酸成分及炮制对两大类成分的影响,探讨山茱萸补肝肾的物质基础。方法:采用OPA-FMOC-C1联用柱前衍生化-RP-HPLC法测定氨基酸的含量及依据JY/T015-1996电感耦合等离子体原子发射光谱方法通则测定溶出液中无机元素的含量。结果:山茱萸生品溶出液中检出十八种无机元素,溶出液富含K,Ca,Mg等元素。炮制后除Cu明显下降、Ba,Ni基本不变外,其他各种元素均有不同程度的增加,稀土元素La,Ce等元素含量增大明显。山茱萸药材检出十六种氨基酸,富含天冬氨酸和谷氨酸。山茱萸酒制后游离氨基酸的总量降低,但赖氨酸、苯丙氨酸等含量增大,炮制后除蛋氨酸、组氨酸遭到破坏,脯氨酸含量显著降低,其余各总氨基酸含量均增大。结论:山茱萸富含天冬氨酸和K,以及多种人体所必须的氨基酸和微量元素,炮制使元素的溶出明显增大,多种氨基酸含量发生改变,赖氨酸、亮氨酸和缬氨酸等肝肾有关的人体必须氨基酸含量显著增大,这些可能是山茱萸炮制后补肝肾作用增强的机制之一。Objective： To investigate amino acid and inorganic element in comus officinalis and study the effects of processed on contents of them. Methods ： The amino acid analysis with precolume derivatization was implemented by HPLC and the contents of element in the prevention were determined by ICE Results： comus officinalis contained eighteen inorganic element and abundant K, Ca, Mg. After processed, except for Cu, Ba, Ni, all were increased and the contents of La and Ce were added remarkably. Sixteen amino acid were determined in comus officinalis and rich asp and glu were measurated. The total content of amino acid decreased, but contents of lys, phe and etc. increased. After processed, the contents of met, his were destroyed and pro reduced, however the total content of amino acid increased. Conclusion： cornus officinalis contains many types of amino acid and inorganic element. Processing lead to the dissolved of inorganic element increase and change of contents of amino acid. Plenty K, asp and the increase of the contents of lys, leu, val, and etc. being necessary for mamkind may be one of mechanism of enganced effect on protecting liver and kidney.

关 键 词：山茱萸 宏微量元素 氨基酸 炮制 

分 类 号：R283[医药卫生—中药学]