家蝇抗菌肽的分离纯化及生物学活性  

Purification and biological activities of a antibacterial peptides from housefly

作　　者：屈军梅[1] 黄庭汝[2] 屈孝初[1] 李文平[1] 陈平洁[2] 

机构地区：[1]湖南农业大学动物科技学院,湖南长沙410128 [2]广东省农业科学院畜牧研究所,广东广州510640

出　　处：《中国兽医学报》2007年第3期387-390,共4页Chinese Journal of Veterinary Science

基　　金：广东省自然科学基金资助项目(032019)

摘　　要：将诱导家蝇提取的家蝇抗菌肽提取物，经凝胶过滤层析和离子交换层析，得到具有抗菌活性的单一峰，质谱结果显示抗菌肽分离物含有4种小肽，相对分子质量678．3～1017．2。家蝇抗菌肽对革兰氏阴性菌（大肠杆菌、巴氏杆菌）的抗菌活性优于革兰氏阳性菌（金黄葡萄球菌、枯草芽孢杆菌），对耐药性巴氏杆菌有较强的抗菌活性，对大肠杆菌的MIC为4．2～8．4mg／L，对耐药性巴氏杆菌的MIC为3．8～7．8mg／L。扫描电镜观察发现，家蝇抗菌肽通过损伤细胞壁使细胞质外流而达到杀菌的目的。家蝇抗菌肽对家兔、鸡、猪、小鼠、番鸭、鹅和家鸭红细胞无溶血反应和凝集反应。‘After antimicrobial peptide extracts induced and extracted from housefly （Musca domestica） were separated by sephadex G-50 and CM-sepharose, a single hill with antimicrobial activity was obtained. By I,C/MS/ MS analysis, it was showed that purification contained 4 peptides of 678.3-1 017.2. Antimicrobial peptide whose activities against gram-positive were better than against gram-negative bacteria, had strong activity againt P. rnultocida with drug resistance. MIC of the peptide were 4.2-8.4 mg/L on Escherichia coli, and 3.8-7.8 mg/L on P. rnultocida. It was found by scanning electron microscope that bacteriocidal actions were accomplished through injurying cell membrane and making cytoplasm flowing out. There were no hemolysis and hemagglution of peptide to red cells of rabbit, chick, pig, rat, muscovy duck, goose and duck.

关 键 词：蒙蝇 抗菌肽 生物学活性 质谱 

分 类 号：S852.74[农业科学—基础兽医学]