喉癌原代培养细胞及Hep-2细胞系染色体畸变研究  

Analysis of chromosome aberrations in the cell derived from primary cell culture of laryngeal carcinoma and the Hep-2 cell line

作　　者：康宁[1,2] 李福才[1] 富伟能[1] 张景海[2] 孙开来[1] 

机构地区：[1]中国医科大学基础医学院医学遗传学教研室,沈阳110016 [2]沈阳药科大学生物化学与分子生物学教研室

出　　处：《中华医学遗传学杂志》2007年第2期131-135,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30171008）

摘　　要：目的探索原发性喉鳞状细胞癌及喉癌Hep-2细胞系的特征性染色体异常，认识喉癌的细胞遗传学改变与其发病机理的相关性。方法对喉癌手术新鲜标本进行改良的原代细胞培养，G显带后核型分析；应用高分辨染色体分析法对喉癌Hep-2细胞系进行核型分析；应用6号染色体涂染探针对原发性喉癌及喉癌细胞系进行分子细胞遗传学研究。结果4例原发性喉癌原代培养细胞中，1例是四倍体范围，3例是三倍体范围。Hep-2细胞系染色体众数为68～75条，出现15条可识别的标记染色体。原发性喉癌及喉癌细胞系中染色体结构异常多为末端缺失、等臂染色体和不平衡易位，而且均存在复杂的6号染色体畸变。结论6q-，i（5p），17p-，5q-可能是人类喉鳞状细胞癌特征性染色体改变；荧光原位杂交法（fluorescence in situ hybridization，FISH）可以探求常规显带方法难以确认的复杂易位和标记染色体来源，为进一步确定喉癌特征性染色体畸变提供有价值的依据。Objective To search for characteristic chromosome changes in primary laryngeal squamous cell carcinoma（LSCC） and Hep-2 cell hne and to realize the relationship between the cytogenetic abnormahty and the pathogenetic mechanism in LSCC. Methods The fresh resulted samples of LSCC were analyzed with an improved primary cell culture for chromosome preparation and G-banding technique. Hep-2 cell line was analyzed by high resolution banding technique. Molecular cytogenetics analysis was made by chromosome 6 painting probe. Results Four primary LSCC succeeded in primary cell culture and obtained metaphases, one was tetraploid, the other three were triploid. The chromosome mode of Hep-2 cell hne was from 68 to 75 and fifteen marker chromosomes were found. The most structural abnormalities of chromosome in primary LSCC and HEP-2 cell line were unbalance translocation, terminal deletion and isochromosome. The comphcate aberration in chromosome 6 was common in LSCC and Hep-2. Conclusion 6q- , i （5p） ,17p- ,5q- are considered as characteristic chomosome changs in LSCC. Fluorescence in situ hybridization （FISH） may enhance the ability of detecting comphcated chromosome rearrangements and marker chromosomes, which could provide more value data to verify the chromosome characteristic aberration in LSCC.

关 键 词：喉鳞状细胞癌 细胞遗传学 荧光原位杂交 染色体畸变 

分 类 号：R686[医药卫生—骨科学]