快速眼动睡眠剥夺后大鼠脑内神经元骨架蛋白及超微结构的变化  

Rapid eye movement sleep deprivation induces changes of neuronal cytoskeleton protein and ultrastructure in rats

作　　者：田国红[1] 黄建欧[1] 赵忠新[1] 张琳[1] 

机构地区：[1]第二军医大学附属长征医院神经内科,上海200003

出　　处：《中华神经科杂志》2007年第4期270-274,共5页Chinese Journal of Neurology

基　　金：国家自然科学基金(30270487);上海市科技发展基金资助项目(024119029)

摘　　要：目的探讨经历不同时间快速眼动(REM)睡眠剥夺对大鼠皮质及海马各区神经元形态结构的影响。方法选择微管相关蛋白(MAP2)和神经丝(NF)作为正常神经元结构的标识物,利用免疫组织化学法和 Western blot 技术观察 REM 睡眠剥夺1、3、5、7 d 4个时间点大鼠皮质及海马MAP2和 NF 表达的时空变化规律。同时运用电镜技术观察睡眠剥夺后神经元超微结构的变化。我们的实验是用改良的多平台睡眠剥夺模型进行 REM 睡眠剥夺,结合免疫组织化学染色技术和蛋白质电泳以及电镜超微结构分析。结果 REM 睡眠剥夺后5 d 大鼠皮质、海马 CA_1及齿状回神经元结构蛋白 MAP2和 NF 表达较对照组明显减少(P<0.05);电镜神经元核仁偏位,胞质中出现少量肿胀的线粒体和内质网;部分神经轴突的髓鞘溶解与浓集。环境对照组、REM 睡眠剥夺5 d 和7 d 组,皮质中超微结构改变的神经元所占比例分别为1.2%、3.6%和5.8%。结论 REM 睡眠剥夺能够导致大鼠脑内神经元的超微结构发生异常变化。Objective To investigate the impact of rapid eye movement （REM） sleep on the rat brain. Methods Microtubule-associated protein 2 （MAP2） and neurofilament （NF） were used as two neuronal cytoskeleton markers. The modified multiple platform method （ MMPM ） was used to deprived rats of sleep. The immunohistochemistry method and Western blot technique were used to detect the expression of MAP2 and NF, together with the electromicroscope study to show the neurodegeneration in rat cerebral cortex and hippocampus. Results The expression of MAP2 and NF in cerebral cortex and hippocampal neurons decreased after 5-day sleep deprivation. Some ultrastructural changes were seen in those areas as well, such as displacement of the nucleus, slightly swollen mitochondrial and endoplasmic reticulum, dissolution or condensation of sheaths of some axons. Neurons in cortex with degenerated ultrastrutrue accounted for 1.2%, 3.6% and 5. 8% in tank control group, sleep deprivation 5 d group and sleep deprivation 7 d group respectively. Conclusion REM sleep deprivation can induce neurodegeneration in rat brain, but these morphological changes are tiny enough to be considered as reversible.

关 键 词：睡眠剥夺 微管相关蛋白质类 中间丝 显微镜检查 电子 

分 类 号：R741[医药卫生—神经病学与精神病学]