稻属谷氨酰胺合成酶家族的系统分析  

Comparative Phylogenetic Analysis of Glutamine Synthetase Family in Oryza

作　　者：王江[1] 张景六[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《植物生理学通讯》2007年第2期277-282,共6页Plant Physiology Communications

基　　金：国家高技术研究发展计划"(863"计划;2006AA10A102)。

摘　　要：比较籼粳栽培稻和野生稻中谷氨酰胺合成酶(GS)基因和蛋白质的结果表明,水稻GS蛋白编码区序列高度保守,而非编码序列变异较大。GS2基因的进化比GS1基因保守。短药野生稻中GS基因进化主要是内含子的变异,但此种内含子的变异在籼粳栽培稻中幅度要小得多。Glutamine synthetase （GS, EC 6.3.1.2） is a key enzyme that catalyzes the conversion of NH4^＋ in plants. In this paper, we compared GS in the level of DNA and protein in two subspecies （indica and japonica） of cultivated rice （Oryza sativa） and wild rice （Oryza brachyantha）. The results suggested that the coding sequences of GS gene were highly conserved, but non-coding sequences had a large range of variation. In wild rice, the evolution of GS gene could be mainly induced by the variation from introns, but which appeared in lower extent between two cultivated subspecies （indica and japonica）.

关 键 词：谷氨酰胺合成酶 短药野生稻 栽培稻 序列比对 分子进化树 

分 类 号：S511[农业科学—作物学]