Effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow, gastric motiiity and brain-gut peptide  

作　　者：Ya-Ping Lin Shou-Xiang Yi Jie Yan Xiao-Rong Chang 

机构地区：[1]The Institute of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China

出　　处：《World Journal of Gastroenterology》2007年第15期2229-2233,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the Major State Basic Research Development Program of China (973 program), No. 2005-CB523306-9

摘　　要：AIM： To observe the effect of acupuncture at Foot- Yangming Meridian on gastric mucosal blood flow （GMBF）, gastric motility and brain-gut peptide. METHODS： Sixty SD rats were randomly divided into 6 groups： normal control group, model group （group with gastric mucosal damage, GMD）, Sibai group （with acupuncture at Sibai point ＋ GMD）, Tianshu group （with acupuncture at Tianshu point ＋ GMD）, Zusanli group （with acupuncture at Zusanli point ＋ GMD） and non-acupoint group （with acupuncture at non-acupoint ＋ GMD）. The GMD model group was induced by infusing pure alcohol into gastric cavity. H2 Gas Clearance Test （HGCT） was used to measure GMBF, the frequency and amplitude of gastric motility were measured by the method of aerocyst, the content of brain-gut peptide in sinus ventriculi and bulbus medullae were detected by radioimmunoassay. RESULTS： Inhibitory effect of the frequency and amplitude of gastric motility were shown in model group, and the rates of frequency and amplitude changes were remarkably different from the normal control group （-19.41 ± 17.21 vs-4.71 ± 10.32, P 〈 0.05; -51.61 ± 29.02 vs 1.81 ± 14.12, P 〈 0.01）. In comparison with control group, the GMBF was 0.52 ± 0.161 mL vs 1.03 ± 0.255 mL per 100g tissue/min, P 〈 0.01, the content of motilin in sinus ventriculi and bulbus medullae was 63.04 ± 7.77 pg/mL vs 72.91 ± 8.42 pg/mL, P 〈 0.05 and 50.96 ± 8.77 pg/mL vs 60.76 ± 8.05 pg/mL, P 〈 0.05, but the content of somatostatin in sinus ventriculi and bulbus medullae was 179.85 ± 43.13 ng/g vs 90.54 ± 40.42 ng/g, P 〈 0.01 and 532.86 ± 122.58 ng/g vs 370.91 ± 76.29 ng/g, P 〈 0.05,respectively. In comparison with model group, the amplitude of gastric motility was 1.52 ± 20.13, -6.52 ± 23.31, 6.92 ± 25.21 vs -51.61 ± 29.02, P 〈 0.01 and GMBF was 0.694 ± 0.160 mL vs 0.893 ± 0.210 mL, 1.038 ± 0.301 mL vs 0.52 ± 0.161 mL per 100g tissue/rain, P 〈 0.01, respectively in Tianshu, Sibai and Zusanli groups, the content of motilin 瞄准：在胃的粘膜血流(GMBF ) 上在 Foot-Yangming 顶点观察针灸的效果，胃的活动性和大脑勇气肽。方法：六十只 SD 老鼠随机被划分成 6 个组：正常控制组，为组建模(有胃的粘膜损坏的组， GMD )， Sibai 组(在在 Sibai 点+ GMD 的针灸方面)， Tianshu 组(在在 Tianshu 点+ GMD 的针灸方面)， Zusanli 组(在在 Zusanli 点+ GMD 的针灸方面)并且 non-acupoint 组(在在 non-acupoint + GMD 的针灸方面)。GMD 模型组被灌输纯酒精进胃的洞劝诱。H2 煤气的清理测试(HGCT ) 被用来测量胃的活动性的 GMBF，频率和振幅被飞行器的包囊的方法测量，在湾穴 ventriculi 和灯泡的大脑勇气肽的内容我们髓被放射性免疫测定检测。结果：频率的禁止的效果和胃的活动性的振幅在模型组被显示出，并且频率的率和振幅变化与正常控制组显著地不同(- 19.41 +/- 17.21 对 -4.71 +/- 10.32， P < 0.05；-51.61 +/- 29.02 对 1.81 +/- 14.12， P < 0.01 ) 。与控制组比较， GMBF 是 0.52 +/- 0.161 mL 对 1.03 +/- 0.255 mL 每 100g tissue/min ， P < 0.01 ，在湾穴 ventriculi 和灯泡的 motilin 的内容我们髓是 63.04 +/- 7.77 pg/mL 对 72.91 +/- 8.42 pg/mL ， P < 0.05 和 50.96 +/- 8.77 pg/mL 对 60.76 +/- 8.05 pg/mL ， P < 0.05 ，但是在湾穴 ventriculi 和灯泡的 somatostatin 的内容我们髓是 179.85 +/- 43.13 ng/g 对 90.54 +/- 40.42 ng/g ， P < 0.01 和 532.86 +/- 122.58 ng/g 对 370.91 +/- 76.29 ng/g ， P < 0.05 分别地。与模型组比较，胃的活动性的振幅是 1.52 +/- 20.13 ， -6.52 +/- 23.31 ， 6.92 +/- 25.21 对 -51.61 +/- 29.02 ， P < 0.01 并且 GMBF 是 0.694 +/- 0.160 mL 对 0.893 +/- 0.210 mL ， 1.038 +/- 0.301 mL 对 0.52 +/- 0.161 mL 每 100g tissue/min ， P < 0.01 ，分别地在 Tianshu ， Sibai 和 Zusanli 组织，在湾穴 ventriculi 和灯泡的 motilin 的内容我们髓是 71.64 +/- 9.35 pg/mL 对 63.04 +/- 7.77 pg/mL ， P < 0.05 ； 58.96 +/- 6.69

关 键 词：ELECTRO-ACUPUNCTURE Foot-Yangming Meridian Gastric motility MOTILIN SOMATOSTATIN 

分 类 号：R245.3[医药卫生—针灸推拿学] R245-0[医药卫生—中医临床基础]