源于细菌CpG基序的寡核苷酸激活单核/巨噬细胞抗白血病效应的研究  

Effect of monocyte/macrophages activated by CpG-oligodeoxynucleotides of bacteria on K562 cells

作　　者：陈春燕[1] 王红艳[1] 曹倩[1] 肖颖[1] 王琳琳[1] 张荣梅[2] 贾继辉[1] 

机构地区：[1]山东大学医学院微生物学教研室,山东济南250012 [2]山东大学第二医院血液-肿瘤科,山东济南250033

出　　处：《中国病理生理杂志》2007年第5期982-985,共4页Chinese Journal of Pathophysiology

基　　金：山东省优秀中青年科学家奖励基金资助项目(No2004BS02007);山东省科技攻关重点资助项目(No2004GG2202107);济南市青年科技明星计划资助项目(No50114);山东大学创新团队计划项目资助项目

摘　　要：目的:研究源于细菌CpG基序的寡核苷酸激活单核/巨噬细胞抗白血病细胞的作用。方法:用血细胞分离机从健康人外周血分离并诱导出单核-巨噬细胞,流式细胞仪检测细胞表面CD14分子和CD16分子表达状况。设计合成含CpG基序的寡核苷酸(CpG-ODN)和不含CpG基序的寡核苷酸(nonCpG-ODN)分别作用于单核/巨噬细胞,MTT法检测经寡核苷酸作用后,单核/巨噬细胞抗白血病K562细胞的效应,用ELISA法检测其分泌细胞因子IL-12、TNF-α的表达。结果:从健康人外周血分离并成功诱导出单核/巨噬细胞,证实CpG-ODN作用于单核/巨噬细胞,可显著增强单核/巨噬细胞体外抗白血病细胞的作用,同时能促进单核/巨噬细胞分泌细胞因子IL-12、TNF-α。结论:源于细菌CpG-ODN可增强单核/巨噬细胞介导的抗白血病细胞作用,此为白血病免疫治疗提供了新的途径。AIM： To study the effect of monocyte/macrophages treated with CpG - oligodeoxynucleotides on leukemic K562 cells. METHODS： The monocytes/macrophages from peripheral blood cells were isolated and induced. The expressions of CD14 and CD16 on monocytes/macrophages were detected by means of flow cytometry. After treated with synthetic CpG - oligodeoxynucleotides, and nonCpG - oligodeoxynucleotides for 24 hours respectively, the inhibiting effect of monocyte/macrophages on K562 cells were detected using MTT method. The secretions of TNF -α and IL - 12 from monocytes/macrophages were determined using ELISA method. RESULTS： The monocytes/macrophages treated with CpG - oligodeoxynucleotides enhanced their antitumor effect on K562 cells and increased the secretion levels of TNF - α and IL - 12. Whereas, there was no significant difference between antitumor effect and cytokine secretion of the monocytes/ macrophages treated with nonCpG- oligodeoxynucleotide. CONCLUSION： CpG- oligodeoxynucleotides increases the cytotoxicity of macrophages on K562 cells in vitro, as well as facilitates the IL- 12 and TNF -α secretion. It provides a new approach for immunologic treatment of leukemia.

关 键 词：寡核苷酸类 单核细胞 巨噬细胞 白血病 K562细胞 

分 类 号：R363[医药卫生—病理学]