碱性成纤维细胞生长因子对大鼠脑血肿周围组织和海马bax及bcl-2基因表达的调节  

Regulatory effects of basic fibroblast growth factor on the expressions of bax and bcl-2 genes in the perihematoma and hippocampus tissues after intracerebral hemorrhage in rats

作　　者：黄俊杰[1] 李倩茗[1] 韦红巧[1] 黄巨恩[2] 李炳[3] 

机构地区：[1]广西医科大学生理学教研室,广西壮族自治区南宁市530021 [2]广西医科大学组胚学教研室,广西壮族自治区南宁市530021 [3]新乡医学院生理学教研室,河南省新乡市453000

出　　处：《中国组织工程研究与临床康复》2007年第14期2637-2640,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广西科学基金(桂科回0575013)~~

摘　　要：目的:观察碱性成纤维细胞生长因子对大鼠脑出血后出血灶周围脑组织和出血侧海马bax、bcl-2基因表达的影响,探讨神经营养因子对神经细胞调亡的调控。方法:实验于2006-01/10在广西医科大学医学科学实验中心完成。①取成年清洁级Wistar大鼠72只,雌雄各半,体质量250g左右。②采用脑内囊注射胶原酶建立大鼠脑出血模型,动物于苏醒后按Bederson法进行神经病学评分,评分>3分后入选本实验,入选72只大鼠随机抽签法分为3组,每组24只。碱性成纤维细胞生长因子组按8μg/kg剂量肌肉注射,1次/d;生理盐水组肌肉注射等剂量的生理盐水,1次/d;模型组不作任何干预。③每组分别于干预后1,3,7d随机抽取8只大鼠,麻醉状态下取出血灶周围脑组织和出血侧海马,采用半定量反转录-聚合酶链反应检测调亡调控基因bax mRNA,bcl-2mRNA的表达。结果:在建立脑出血模型中共5只大鼠死亡,随后对死亡动物进行解剖,发现脑内血肿量过大,致脑疝形成而导致死亡,后随机补充动物。72只大鼠进入结果分析。①血肿周围脑组织bax mRNA表达:干预后3d,7d,碱性成纤维细胞生长因子组血肿周围脑组织bax mRNA表达比生理盐水组明显减少(3d:0.54±0.19,0.76±0.23,P<0.05;7d:0.45±0.19,0.71±0.16,P<0.01)。②血肿周围脑组织bcl-2mRNA表达:干预后3d,7d,碱性成纤维细胞生长因子组的血肿周围脑组织bcl-2mRNA表达比生理盐水组明显增高(3d:0.68±0.25,0.39±0.19,P<0.05;7d:0.80±0.21,0.48±0.18,P<0.01)。③出血侧海马bax mRNA表达:干预后3d和7d,碱性成纤维细胞生长因子组的出血侧海马bax mRNA表达比生理盐水组均明显减少(3d:0.54±0.18,0.70±0.11;7d:0.43±0.24,0.69±0.18,P均<0.05)。④出血侧海马bcl-2mRNA表达:干预后3d和7d,碱性成纤维细胞生长因子组的出血侧海马bcl-2mRNA表达比生理盐水组均明显增多(3d:0.66±0.11,0.50±0.15;7d:0.72±0.12,0.52±0.22,P均<0.05)。结论:碱性成纤维细胞�AIM： To observe the effects of basic fibroblast growth factor （bFGF） on the expressions of bax and bcl-2 genes in the perihematoma and hippocampus tissues in rats with intracerebral hemorrhage （ICH）, and explore the regulatory effect of neurotrophic factor on nerve cell apoptosis. METHODS： The experiment was conducted in the Experiment Center of Medical Science, Guangxi Medical University from January to October 2006. ①Seventy-two clean grade adult Wistar rats with half male and female and 250 g were selected. ②ICH models were established by injection of collagenase into internal capsule of cerebrum. The neuroethological scores were detected by Bederson method to choose the rats with more than 3 points. The enrolled 72 Wistar rats were randomly divided into three groups with 24 rats in each group, bFGF treatment group was intramuscularly injected with 8 μg/kg bFGF once daily; normal saline group was given matching normal saline once daily; model group was not treated. ③Eight rats from each group were selected at 1, 3 and 7 days, respectively to harvest the perihematoma and hippocampus tissues under anesthesia. The expression levels of bax and bcl-2 genes were detected by RT-PCR. RESULTS： Five rats died of cerebral hernia caused by over intracerebral hematoma during modeling. Seventy-two rats were involved in the result analysis after supplement. ①bax mRNA expression around perihematoma tissue： After 3 and 7 days intervention, the expression level of bax gene of the bFGF group were significantly decreased compared with the normal saline group （3 days： 0.54±0.19, 0.76±0.23, P 〈 0.05; 7 days： 0.45±0.19, 0.71±0.16, P 〈 0.01）. ②bcl-2 mRNA expression around perihematoma tissue： After 3 and 7 days intervention, the expression level of bcl-2 gene of the bFGF group were significantly increased compared with the normal saline group （3 days： 0.68±0.25, 0.39±0.19, P 〈 0.05; 7 days： 0.80±0.21, 0.48±0.18, P〈 0.01）. ③bax mRNA expression in hippocampus tissue：

关 键 词：成纤维细胞生长因子2 脑出血 原癌基因蛋白质类 逆转录酶聚合链反应 组织构建 

分 类 号：R743.34[医药卫生—神经病学与精神病学]