用糖基化磷脂酰肌醇B7-1锚定肿瘤细胞膜进行免疫治疗  

Glycosyl-phosphatidylinositol-B7-1 anchors into tumor cell membranes:a novel approach to tumor immunotherapy

作　　者：张淑敏[1] 畅继武[1] 马富玲[1] 隋志方[1] 王馨[1] 张新[1] 刘凤勇[1] 

机构地区：[1]天津市泌尿外科研究所肿瘤免疫室,天津300211

出　　处：《免疫学杂志》2007年第3期323-326,共4页Immunological Journal

基　　金：卫生部默沙东科研基金;天津市科委基金资助项目(003111711)

摘　　要：目的研制抗肿瘤免疫治疗的新疫苗。方法用蛋白转化法将B7-1锚定在肿瘤细胞膜上,免疫C57BL-6小鼠后,一组小鼠取脾细胞进行T细胞扩增和细胞毒T淋巴细胞(CTL)功能检测,另一组小鼠进行肿瘤细胞接种试验。结果用GPI-B7-1修饰的肿瘤细胞膜免疫小鼠后诱发了肿瘤特异性T细胞扩增和CTL。且该疫苗有一定的保护小鼠免受肿瘤细胞侵袭的作用。结论GPI蛋白转化法为人类抗肿瘤免疫治疗提供了新的、有效的修饰肿瘤细胞膜的方法。Objective To develop therapeutic cancer vaccines for use in human immunotherapy. Methods Stable incorporation of B7-1 on tumor cell membranes was achieved by incubating the isolated tumor membranes with purified GPI-B7-1. C57BL/6 mice were immunized with the modified tumor membranes, and then spleens of some mice were harvested for T cell proliferation and CTL assays. The other immunized mice were injected RM-1 cells for tumor challenge experiments. Results Immunization of C57BL/6 mice with the modified RM-1 tumor membranes induced tumor-specific T-cell proliferation and CTLs. In addition, a ceaain extent immunization with these RM-1 membranes could protect mice from parental tumor challenge. Conclusion Protein transfer of glycolipid-anchored molecules provides an novel and efficient approach to modify tumor membranes for human immunotherapy.

关 键 词：GPI-B7-1 肿瘤细胞膜 免疫治疗 

分 类 号：R730.5[医药卫生—肿瘤]