抗菌类药物对锯缘青蟹N-乙酰-β-D-氨基葡萄糖苷酶活力的影响  

Effects of antibacterial medicaments on the activity of N-acetyl-β-D-glucosaminidase from mud crab(Scylla serrata)

作　　者：颜雅雯[1] 张继平[1] 王勤[1] 谢进金[2] 陈清西[1] 

机构地区：[1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]泉州师范学院生物系,福建泉州362000

出　　处：《台湾海峡》2007年第2期231-235,共5页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金资助项目(40576066);厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室资助项目(2005101)

摘　　要：研究了几种抗菌类药物对锯缘青蟹(Scylla serrata)N-乙酰-β-D-氨基葡萄糖苷酶(简称NAGase,EC 3.2.1.52)活力的影响.其结果表明:链霉素、卡那霉素、青霉素钾、庆大霉素和磺胺嘧啶对该酶活力均没有明显的影响.头孢拉定对该酶有显著的激活作用,当其浓度为4.5mg/cm3时,可使酶活力提高200%.而氟哌酸和恩诺沙星对该酶均有抑制作用,其中恩诺沙星对该酶的抑制作用较强,随着抑制剂浓度增大,酶活力呈指数下降,导致酶活力下降50%的抑制剂浓度(IC50)为0.44mg/cm3.恩诺沙星对该酶的抑制作用类型表现为非竞争性可逆过程,抑制常数KI为0.35mg/cm3.该研究对锯缘青蟹的人工养殖具有一定的参考价值.The effects of antibacterial medicaments on the activity of N-acetyl-β-D-glucosaminidase （NAGase） from mud crab （Scylla serrata） were studied. The results showed that streptomycin, kanamycin, benzylpeniciUic, gentamicin and sulfadiazine a effected lightly on the enzyme activity, while cefradine activated the enzyme obviously and enhanced the enzyme activity by 200% in concentration of 4.5mg/cm^3. Norfloxacin and enroflxacin inhibited the enzyme activity. Enroflxacin, particularly, was a potential potent inhibitor, and the inhibitor＇s concentration leading to 50% of enzyme activity lost （IC50） was estimated to be 0. 44mg/cm^3. The inhibitory kinetics and mechanism of enroflxacin on the enzyme were studied. It showed that enroflxacin was a reversible non-competifive inhibitor of the enzyme with an inhibitory constant （K1） of 0. 35mg/cm^3.

关 键 词：锯缘青蟹 N-乙酰-Β-D-氨基葡萄糖苷酶 抗菌类药物 酶活力 抑制作用 

分 类 号：Q356.1[生物学—遗传学]