滇产与日产松茸的IGS1-RFLP比较分析  

IGS1-RFLP Comparative Analysis of Tricholoma matsutake (Tricholomataceae) from Yunnan and Japan

作　　者：沙涛[1] 钱勇[2] 张汉波[1] 丁骅孙[2] 程立忠[2] 赵之伟[1] 

机构地区：[1]云南大学生物资源保护与利用国家重点实验室培育基地,云南昆明650091 [2]云南大学生物学系,云南昆明650091

出　　处：《云南植物研究》2007年第2期243-246,共4页Acta Botanica Yunnanica

基　　金："十五"国家科技攻关计划"生物资源与生物安全技术研究开发"项目(No.2001BA707B01);云南省应用基础研究基金(2005C0003M)

摘　　要：应用Cfr13I限制性内切酶对采自于云南省16个市县的127个松茸子实体进行了IGS1-RFLP比较分析,发现126个松茸子实体属于A类型,来自大理白族自治州剑川县的1例(TF89)为C类型。通过对A类型的IGS1序列分析发现产自云南的松茸有一个CTTT的简单重复,滇产松茸IGS1序列差异不明显。滇产松茸的IGS1-RFLP与日产松茸的主要类型十分相似,两地松茸可能是同源的。IGS1 genes were obtained by PCR amplification from genome DNA of Tricholoma matsutake fruit bodies collected from 16 counties in Yunnan. These gene fragments were digested by restriction endonuclease Cfr13 I . Two distinct IGS1 rDNA types were identified, namely A and C. Among the 127 fruit bodies investigated, 126 belong to type A while one from Jianchuan （T89） belongs to type C. Our results also showed that there are no significant heterogeneity in the IGS1 genes of these fruit bodies, except double CTITs found in representative IGS1 gene of type A. The primary IGS1 RFLP patterns of Tricholoma matsutake from Yunnan are very similar to those from Japanese, implying that Tricholoma matsutake of these two areas are likely to be homologous.

关 键 词：松茸 云南 IGS1-RFLP酶切类型 IGS1序列 

分 类 号：Q943[生物学—植物学] S646[农业科学—蔬菜学]