利用组织芯片技术研究P73和mP53基因编码蛋白在胃癌中的表达及意义  

Clinicopathological significances of P73 and P53 protein expression in gastric carcinomas with tissue microarray technique

作　　者：林志[1] 辛彦[2] 吴东瑛[2] 

机构地区：[1]辽宁省肿瘤医院内三科,辽宁省沈阳市110014 [2]中国医科大学附属第一医院肿瘤研究所第四研究室,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2007年第10期1087-1091,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30371607;教育部博士点专项基金资助项目.No.20040159021~~

摘　　要：目的:构建胃癌及其癌前病变组织芯片,采用免疫组化方法观察研究P73和mP53基因编码蛋白在胃癌中的表达并探讨其临床病理学意义．方法:收集2003-2004年辽宁省肿瘤医院和中国医大附属一院104例胃癌及癌前病变组织标本构建两个组织芯片蜡块,组织样品直径为1 mm．采用SABC免疫组化方法检测胃癌组织中P73和mP53蛋白的表达,观察分析其与胃癌病理生物学行为的关系．结果:P73基因编码蛋白在胃癌、肠上皮化生、不典型增生病变组织中的阳性表达率显著高于远癌正常胃黏膜(90．1％,44．0％,80．0％vs 17．9％,P<0．01)．BorrmanⅢ／Ⅳ型胃癌P73蛋白阳性表达率(92．9％／100％)显著高于BorrmanⅡ型胃癌(57．1％)(P<0．05)．伴转移胃癌组P73蛋白阳性表达率(淋巴结转移组94．4％,肝转移组100％,卵巢转移100％)显著高于无转移组(76．2％)(P<0．05)．胃癌组织中P73蛋白表达与mP53蛋白表达密切相关(x2=9．6736,P<0．01).结论:P73蛋白表达与胃癌恶性病理生物学行为密切相关,其虽与抑癌基因P53同源,但与mP53蛋白表达呈正相关,提示其可能作为P53的一种模拟突变体在胃癌发生、发展中起作用．AIM： To investigate the expression of P73 and mutant P53 （mP53） gene coding proteins and their significances in gastric carcinoma, METHODS： Two tissue microarray blocks containing 104 cases of gastric cancer and precancerous lesions （1.0 mm each in diameter） was constructed. SABC immunohistochemical stainning was used to detect the expression of P73 and mP53 proteins and the correlation between their roles and the pathological behavior of gastric carcinoma was investigated. RESULTS： The positive rate of P73 protein was significantly higher in gastric cancer, intestinal metaplasia and atypical hyperplasia than that in the normal tissues （90.1%, 44.0%, 80.0% vs 17.9%, P 〈 0.01）. The expression of P73 protein was also markedly higher in gastric carcinoma of Borrman Ⅲ/ Ⅳ types than that of Borrrnan Ⅱ type （92.9%/100% vs 57.1%, P 〈 0.05）. Moreover, the expression P73 protein was markedly increased in gastric cancer with lymph node metastasis, liver metastasis and ovarian metastasis as compared with that in non-metastatic cancer （94.4%, 100%, 100% vs 76.2%, P 〈 0.01）. There was a positive correlation between P73 and mP53 expression （Χ^2 = 9.6736, P 〈 0.01）. CONCLUSION： P73 expression is correlated with the malignant behaviors of gastric cancer. Although P73 protein has a similar construction to P53 protein, its expression shows a positive correlation with that of raP53 protein. This finding indicates that P73 may play a role in the carcinogenesis of gastric cancer as a mimic mutant of P53.

关 键 词：胃癌 组织芯片 免疫组化 P73 突变型P53 

分 类 号：R735.2[医药卫生—肿瘤]