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机构地区:[1]中国医科大学附属盛京医院胃肠外科,辽宁省沈阳市110004
出 处:《世界华人消化杂志》2007年第10期1096-1102,共7页World Chinese Journal of Digestology
摘 要:目的:观察MTA1,PTEN,E-cadherin蛋白在胃癌和正常胃黏膜组织中的表达,探讨其与胃癌浸润、转移和生物学行为的关系.方法:应用免疫组织化学方法检测54例胃癌手术切除标本和15例正常胃黏膜组织中MTA1,PTEN,E-cadherin的表达.各指标之间相关因素的差异性比较采用x2检验,相关性研究采用Spearman相关分析:结果:与正常胃组织相比,MTA1蛋白在胃癌组织中高表达(46.3%vs6.7%,P<0.01),PTEN和E-cadherin蛋白在胃癌组织中表达下调或缺失(51.9%vs 100%,42.6%vs 100%,均P<0.01).MTA1和PTEN的阳性表达率与肿瘤浸润深度(P=0.003,P=0.001)、病理分期(P=0.004, P=0.008)、淋巴转移(P=0.000,P=0.001)、远隔转移(P=0.004,P=0.006)、临床分期有关(P=0.001,P=0.000);E-cadherin的正常表达率与肿瘤浸润深度(P=0.027)、病理分化程度(P=0.006)、淋巴转移(P=0.044)、临床分期有关(P=0.000).Spearman相关分析显MTA1与PTEN蛋白、MTA1与E-cadherin蛋白的表达呈负相关(r=-0.518,r=-0.424,均P<0.05).PTEN蛋白与E-cadherin蛋白的表达呈正相关(r=0.53,P<0.05).结论:MTA1蛋白水平高表达和PTEN, E-cadherin蛋白水平低表达可能与胃癌浸润和转移有关,且联合检测可以用于判断胃癌的生物学行为.AIM: To investigate the expression of metastasis- associated gene 1 (MTA1), PTEN and E-cadherin in gastric carcinoma and normal gastric mucosa and their relationships with the invasion, metastasis and biological behavior of gastric carcinoma. METHODS: Immunohistochemistry was used to examine the expression of MTA1, PTEN and E-cadherin proteins in gastric carcinoma (n =54) and normal gastric mucosa (n = 15). The data were processed by chi-square test and the correlation was analyzed by Spearman test. RESULTS: In comparison with those in normal gastric mucosa, the expression of MTA1 in gastric carcinoma was significantly higher (46.3% vs 6.7%, P 〈 0.01), and the expression of PTEN and E-cadherin in gastric carcinoma were downregulated or even disappeared (51.9% vs 100%,42.6% vs 100%; both P 〈 0.01). The expression of MTA1 and PTEN were correlated with the invasion depth (P=0.003, P = 0.001), pathological grades (P = 0.004, P = 0.008), lymph node metastasis (P = 0.000, P = 0.001), distant metastasis (P = 0.004, P=0.006) and clinical classification (P= 0.001, P = 0.000), and the normal expression of E-cadherin had relationship with the invasion depth (P = 0.027), pathological grades (P = 0.006), lymph node metastasis (P = 0.044), and clinical classification (P = 0.000). There was a negative correlation between the expression of MTA1 and PTEN as well as between the expression of MTA1 and E-cadherin (r = -0.518, r = -0.424; both P 〈 0.05), but there was a positive correlation between the expression of PTEN and E-cadherin (r = 0.53, P 〈 0.05). CONCLUSION: High expression of MTA1 and low expression of PTEN and E-cadherin may be associated with the invasion and metastasis of gastric carcinoma. Combined examination of three indexes may be used to evaluate the biological behavior of gastric carcinoma.
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