TβRⅡ,MMP-7,TIMP-2表达及在HNPCC侵袭转移中的作用  

Expression of transforming growth factor β receptor Type II, matrix metalloproteinase-7 and tissue inhibitor of metalloproteinase-2 and their roles in the invasion and metastasis of hereditary nonpolyposis colorectal cancer

作　　者：顾国利[1] 魏学明[1] 任力[2] 王石林[1] 胡益云[2] 李德昌[2] 

机构地区：[1]空军总医院普通外科,北京市100036 [2]空军总医院病理科,北京市100036

出　　处：《世界华人消化杂志》2007年第10期1103-1109,共7页World Chinese Journal of Digestology

摘　　要：目的:通过观察TβR II,MMP-7及TIMP-2在HNPCC和散发性大肠癌中的表达,探讨其在HNPCC侵袭转移的生物学行为中的作用．方法:选取1980-05／2005-06空军总医院收治的HNPCC和散发性大肠癌各30例,应用免疫组化SP染色法检测其肿瘤组织中TβRⅡ, MMP-7,TIMP-2的表达情况．结合其相应临床病理资料进行分析TβR II,MMP-7,TIMP-2表达在HNPCC侵袭转移中的作用．结果:TβRⅡ,MMP-7,TIMP-2在HNPCC组的阳性表达率分别为40．0％,46．7％, 63．3％;而在散发性大肠癌组的阳性表达率为73．3％,86．7％,20．0％．散发性大肠癌组中MMP-7(+～++)／TIMP-2(-)比例明显高于HNPCC组．而其MMP-7(-)／TIMP-2(+-++)者则明显少于HNPCC组．TβR II,MMP-7,TIMP-2的阳性表达率与肿瘤的大小和部位无关．而与肿瘤的侵犯深度和转移与否密切相关．侵犯浆膜外组织和有转移者的MMP-7的阳性表达率明显增高,而TβRII,TIMP-2的阳性表达率则明显降低(P<0．05)．在两组大肠癌中TβRⅡ与MMP-7呈正相关(r=0．735,P=0．000;r= 0．792,P=0．000),TIMP-2与TβRⅡ(r=-0．582, P=0．001;r=-0．394,P=0．031)和MMP-7(r= -0．473,P=0．008;r=-0．388,P=0．034)的表达均呈负相关．结论:TβRⅡ,MMP-7,TIMP-2在散发性大肠癌和HNPCC中的表达差异显著．TβR II, MMP-7在散发性大肠癌中的表达率和表达强度明显高于HNPCC,而TIMP-2的表达结果则相反．因此,散发性大肠癌中MMP-7／TIMP-2的比例失衡明显高于HNPCC组．AIM： To investigate the roles of transforming growth factor β receptor Type II （TβRII）, matrix metalloproteinase-7 （MMP-7） and tissue inhibitor of metalloproteinase-2 （TIMP-2） expression in the invasion and metastasis of human hereditary nonpolyposis colorectal cancer （HNPCC）. METHODS： Immunohistochemical staining was used to detect the expressions of TβR II, MMP-7 and TIMP-2 protein in 30 cases of sporadic colorectal cancer （sporadic CRC） and 30 cases of HNPCC, and their corresponding clinical data were analyzed retrospectively. RESULTS： The positive rates of TβRII, MMP-7 and TIMP-2 expression in HNPCC were 40.0%, 46.7% and 63.3% respectively, while in sporadic CRC, the rates were 73.3%, 86.7% and 20.0% respectively. The ratio of MMP-7（＋-＋＋） to TIMP-2（-） in sporadic CRC was higher while that of MMP-7（-） to TIMP-2（＋-＋＋） was lower significantly than that in HNPCC. TβRII, MMP-7 and TIMP-2 expression were closely related to the depth of invasion and lymph node metastasis, but not to the sex of patients, tumor size and position. The positive rate of MMP-7 expression was significantly increased in tissues with extra-chorion invasion and metastasis, but the expression of TβRII and TIMP-2 were markedly decreased （P 〈 0.05）. In HNPCC and sporadic CRC, positive correlation was found between TβRII and MMP-7 expression （r = 0.735, P=0.000; r= 0.792, P=0.000）, while negative correlation existed between TIMP-2 and TβR II expression （r = -0.582, P = 0.001; r= -0.394, P = 0.031） as well as between TIMP-2 and MMP-7 expression （r = -0.473, P = 0,008; r = -0.388, P = 0.034）. CONCLUSION： TβR II, MMP-7 and TIMP-2 are differently expressed in HNPCC and sporadic CRC. The expression rates and intensities of TβR II and MMP-7 are obviously higher in sporadic CRC than those in HNPCC, while the positive expression of TIMP-2 was a reverse situation. So the imbalance of MMP-7/TIMP-2 is increased in sporadic CRC, Down-regulated expression of MMP-7, TβR I

关 键 词：遗传性非息肉病性大肠癌 转化生长因子β 转化生长因子βⅡ型受体 基质金属蛋白酶-7 金属蛋白酶组织抑制因子-2 病理 免疫组化 

分 类 号：R735.3[医药卫生—肿瘤]