散发性结直肠癌微卫星不稳定性及其与临床病理生物学的关系  

Microsatellite instability in sporadic colorectal cancer and its relationship with clinicopathological features

作　　者：杨柏林[1] 谷云飞[1] 赖仁胜[2] 谢玲[2] 金黑鹰[3] 

机构地区：[1]南京中医药大学附属医院肛肠科,江苏省南京市210029 [2]南京中医药大学附属医院病理科,江苏省南京市210029 [3]南京中医药大学第三附属医院全国肛肠医疗中心,江苏省南京市210001

出　　处：《世界华人消化杂志》2007年第10期1160-1164,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30572447~~

摘　　要：目的:探讨分析中国人散发性结直肠癌微卫星不稳定的变异及其与临床病理生物学特征的关系．方法:应用荧光多重PCR方法检测105例散发性结直肠癌初诊患者微卫星状态,分析MSI结直肠癌潜在的相关临床病理生物学特征．结果:105结直肠癌中,MSI检出率24．7％,其中MSI-H 14例(13．3％),MSI-L 12例(11．4％);队列中各位点突变率分别是D5S346(5．7％), BAT26(8．6％),BAT25(10．5％),D17S250(8．6％), D2S123(10．5％);MSI结直肠癌的组织分化程度与淋巴结转移情况与MSS结直肠癌有显著的统计学差异(P=0．047,P=0．029),但在患者年龄、肿瘤位置、病理性质无显著临床意义(P>0．05)．结论:MSI结直肠癌具有低分化癌多见,淋巴结转移少等特点,淋巴结转移少可能是MSI结直肠癌具有生存优势的原因之一．AIM： To analyze the relationship between microsatellite instability （MSI） and clinicopathological features in sporadic colorectal cancer and its clinical significance. METHODS： Fluorescence multiplex polymerase chain reaction was used to detect the microsatellite instability with five microsatellite loci in 105 patients with sporadic colorectal cancer. The clinicopathological features characterized by mi-crosatellite instability and microsatellite stability were compared statistically. RESULTS： We found microsatellite instability in 24.7% of sporadic colorectal cancer patients, including 14 （13.3%） cases with MSI-high, 12 （11.4%） cases with MSI-low, and 79 （75.3%） cases with MS-stable （MSS）. The instability of D5S346 was found in 6 cases （5.6%）, BAT-26 in 9 （8.6%） cases, BAT-25 in 11 （10.5%） cases, D17S250 in 9 （8.6%） cases, and D2S123 in 11 cases （10.5%）. The differentiation and lymph node metastasis status between MSI- and MSS-positive colorectal cancer was statistically different （P = 0.047, P = 0.029）, but not the remaining variables （P 〉 0.05）. CONCLUSION： Low differentiation and metastasis are found in MSI-posifive sporadic colorectal cancer, which may be one cause of a favorable survival.

关 键 词：散发性结直肠癌 微卫星不稳定 临床病理生物学特征 

分 类 号：R735.3[医药卫生—肿瘤]