榄香烯治疗人喉癌裸鼠模型的实验研究  

Elemene in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice

作　　者：曹忠胜[1] 季文樾[2] 

机构地区：[1]大连医科大学附属第一医院耳鼻咽喉科,大连116011 [2]中国医科大学盛京医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第9期417-420,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:研究榄香烯对人喉鳞状细胞癌细胞株Hep-2细胞荷瘤裸鼠模型移植瘤的生长抑制作用,探讨其抑瘤机制。方法:建立人喉鳞状细胞癌Hep-2细胞株裸鼠皮下移植模型。应用榄香烯治疗,观察移植肿瘤和裸鼠生长情况,计算肿瘤抑制率;逆转录PCR检测肿瘤组织中血管内皮生长因子C(VEGF-C)和血管内皮生长因子受体3(VEGFR-3)mRNA表达情况。结果:榄香烯对人喉鳞状细胞癌细胞株Hep-2细胞荷瘤裸鼠模型移植瘤的增殖具有明显抑制作用,抑瘤率为52.24%。在实验组和对照组之间,鼠净重、瘤重和瘤体积相比,差异有统计学意义(均P<0.01);实验组VEGF-C和VEGFR-3mRNA的表达低于对照组(P<0.01)。结论:榄香烯可显著抑制人喉鳞状细胞癌裸鼠模型肿瘤的生长,其机制与榄香烯抑制VEGF-C和VEGFR-3的表达有关。Objective：To investigate the inhibitory effect of elemene on the tumor growth of human laryngeal squamous carcinoma （LSCC） and to explore the possible mechanism of its inhibitory effect on LSCC. Method： Nude mice model engrafted with laryngocarcinoma was established by using human laryngeal squamous carcinoma cell line （Hep-2）. When the transplantable tumor size is more than 10 mm, the mice were given elemene （120 mg/kg） in the treatment group or PBS in the control group , at the day of 1、4、7、10、13、16、19 and 22 respectively. The animals were killed three days after the last treatment. The tumor volume, tumor weight,mice net weight and tumor inhibition rate were measured. The expression of vascular endothelial growth factor- C （VEGF- C） and vascular endothelial growth factor receptor- 3 （VEGFR- 3） mRNA were quantitated by using reverse transcription and polymerase chain reaction technique. Result： Elemene could inhibit the tumor growth in vivo. The differences were statistically significant for the mice net weight, tumor weight, and tumor volume between the treatment group and the control group. The tumor inhibition percentage was 52.24%. The gene expression of VEGF-C and VEGFR-3 of the treatment group is lower than that of the control group and the differences were statistically significant （ P 〈0.01）. Conclusion： Elemene could inhibit the subcutaneous plantation of human laryngeal carcinoma in nude mice and its mechanism may be associated with inhibited expression of VEGF-C and VEGFR-3.

关 键 词：榄香烯 喉肿瘤 癌 鳞状细胞 血管内皮生长因子C 血管内皮生长因子受体3 

分 类 号：R730.261[医药卫生—肿瘤]