口服耐受后过继转输动物模型的建立方法  

Animal model of adoptive transfer after oral tolerance

作　　者：邓晓静[1] 陈宝安[2] 丁家华[2] 孙雪梅[3] 董伟民[2] 毕延智[2] 张琰[2] 赵刚[2] 高冲[2] 孙耘玉[2] 王骏[2] 程坚[2] 马燕[2] 宋慧慧[2] 鲍文[2] Schmitt A Schmitt M 

机构地区：[1]东南大学临床医学院,江苏南京210009 [2]东南大学附属中大医院血液科,江苏南京210009 [3]南京大学医学院附属鼓楼医院血液科,江苏南京210009 [4]德国乌尔姆大学医学院内科Ⅲ区,德国乌尔姆d89081

出　　处：《东南大学学报（医学版）》2007年第3期161-166,共6页Journal of Southeast University(Medical Science Edition)

基　　金：江苏省135医学工程重点学科项目(K0613);江苏省自然科学基金资助项目(BK1999126);江苏省卫生厅科技发展基金资助项目(H200344);南京市重大课题资助项目(YQ0008);东南大学附属中大医院重大课题资助项目(2003YJ02)

摘　　要：目的:探讨经口服受鼠脾淋巴细胞诱导耐受的供鼠淋巴细胞输注是否能减轻移植物抗宿主病(GVHD)。方法:以雄性DBA-2小鼠(H-2d)为供者,分别用BALB/C鼠脾淋巴细胞、DBA-2鼠脾淋巴细胞、牛血清蛋白、普通饲料隔天喂养,单向混合淋巴细胞反应(MLR)评估诱导耐受状态。以雌性BALB/C小鼠(H-2d)为受者,给予6.0 Gy60Coγ射线全身照射,尾静脉输注来自于DBA-2小鼠的2×107脾淋巴细胞。结果:耐受供鼠cpm值低于非耐受组,接受已耐受供鼠脾淋巴细胞输注的受鼠,发生GVHD较其他组轻。结论:经口服耐受的过继转输动物模型的实验方法有效可行。Objective To investigate whether oral antigens have the effects of amelioration of graft-versus-host disease（GVHD） in mice in donors toward recipient splenocytes. Methods Male （DBA-2）donor mice （H-2^d） were fed with BALB/C splenocytes, DBA-2 splenocytes, bovine serum albumin, or regular chow, every other day. Induction of tolerance was assessed by a mixed lymphocyte reaction（MLR）. Female （BALB/C） mice （H-2^d） as recipients received total body irradiation（TBI） of 6.0 Gy （^60Co γ-ray）followed by injection of 2 ×10^7 splenoeytes supplied by DBA-2. Resuits GVHD was significantly ameliorated in mice as recipients transplanted from tolerized dornors compared with control animals. Conclusion The establishment of adoptive transfer animal model with oral tolerance is feasible and effective.

关 键 词：口服耐受 过继转输 移植物抗宿主病 动物模型 小鼠 

分 类 号：R457.7[医药卫生—治疗学]