庆大霉素C_(1a)单组份产生菌─—棘孢小单孢菌JIM-202的筛选和研究  

Screening and studies on Micromonospora echinospora JIM-202 producer of gentamicin C_(1a)

作　　者：赵敏[1] 范瑾[1] 胡小玲[1] 谢凤龙[1] 刘军[1] 汪洁[1] 杨晓伟[1] 

机构地区：[1]江苏省微生物研究所,无锡214063

出　　处：《中国抗生素杂志》1997年第1期12-15,44,共5页Chinese Journal of Antibiotics

基　　金：国家新药研究基金

摘　　要：利用紫外光和氯化锂复合因子处理小诺霉素产生菌——棘孢小单孢菌JIM－401获得了JIM－202突变株，其发酵产物经阳离子交换树脂提取、洗脱、脱色、浓缩和经冷冻干燥可得白色粉末。用薄层层析检验其发酵产物为1个单组份；通过红外光谱、紫外光谱、核磁共振谱及质谱测定表明该抗生素与庆大霉素C1a同质，因而可用其进行新衍生物合成（如新抗生素89－07）。根据小诺霉素和庆大霉素生物合成途径推测，该突变株在最后一步的6’－N甲基化部位发生了阻断所致。Mcromonospora echinospora JIM-401, a producer of micronomicin wastreated with UV and LiCl. By screening of the mutants with TLC, a mutant which producedonly one component (antibiotic 202) was obtained and named JIM-202. The antibiotic 202was extracted from the fermentation broth by cation ion-exchange resin. The IR, UV, NMRand MS spectra of antibiotic 202 indecated that antibiotic 202 is gentamicin C1a Thus it can bc used as mother nucleuse of new semisynthetic antibiotics(such as antibiotic 89-07). With reference to the biosynthesis pathway of micronomicin andgentamicin. It was assumed that it may be the rcsult of blocking in the step of methylation of6'-N. The cultural characteristic, physiologic-biochemical properties and fermentation ofJIM-202 were also reported.

关 键 词：筛选 诱变筛选 庆大霉素 棘孢小单孢菌 

分 类 号：TQ465.2[化学工程—制药化工]