三七总皂甙对大鼠脊髓损伤后背核和红核神经元存活的影响  

Effects of panax notoginsenosides on neuronal survival in dorsal nucleus of Clarke and red nucleus in injured spinal cord of rat

作　　者：周雯[1] 曾园山[1] 张伟[1] 陈巧格[1] 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室,广东广州510080

出　　处：《解剖学研究》2007年第2期97-100,114,共5页Anatomy Research

基　　金：国家自然科学基金资助项目(30472132);广东省中医药管理局基金(101139;303013)

摘　　要：目的探讨三七总皂甙对脊髓半横断后受损伤背核和红核神经元存活以及背核神经元表达一氧化氮合酶(NOS)的影响。方法25只成年雌性大鼠在施行脊髓半横断术后,被分为对照组、三七总皂甙低剂量组、三七总皂甙中剂量组、三七总皂甙高剂量组和L-NNA组。三七总皂甙组每天胃饲一定剂量的三七总皂甙,L-NNA组每d腹腔内注射L-NNA。术后30d取出脊髓和大脑行冷冻切片,做NADPH酶组化染色和中性红染色。结果脊髓半横断后,对照组损伤侧背核神经元和红核神经元数目明显降低,背核面积减少,背核NOS阳性神经元增多;应用三七总皂甙和L-NNA后,损伤侧背核神经元数目和背核面积有恢复性增加,尤其是高剂量三七总皂甙的效果更明显。三七总皂甙和L-NNA均可抑制受损伤的背核神经元表达NOS,三七总皂甙还可提高损伤侧红核神经元的存活率。结论三七总皂甙能够促进受损伤的背核神经元和红核神经元的存活,同时能够抑制受损伤的背核神经元表达NOS。Objective To explore the effects of panax notoginsenosides （PNS） on the survival of injured neurons in dorsal nucleus of Clarke （CN） and red nucleus （RN） and NOS expression of CN neuron after spinal cord hemi-transsection. Methods 25 adult female rats were divided into the control group, low dosage group of PNS, medium dosage group of PNS, high dosage group of PNS, and L-NNA group after the spinal cord hemi-transsected. Corresponding dosage of PNS was fed to the rats in the three groups of PNS groups every day. L-NNA group was .injected intraperitoneally in the L-NNA group every day. In the 30th day after spinal cord hemi-transsection, the spinal cord and brain, taken from all the rats, were sectioned with cryostat and stained by NADPH enzyme histochemistry and neutral red. Results After spinal cord hemi-transsection, the quantity of CN and RN neurons decreased obviously, the area of CN was diminished, and the NOS positive neurons of CN increased at injured side in the control group. After the administration of PNS and L-NNA, the quality of CN neurons and the area of CN at injured side were recoverly enhanced, especially in the high dosage of PNS group. PNS and L-NNA could equally inhibit the NOS expression of injured neurons of CN. PNS could also promote the neuronal survival of RN at injured side. Conclusion PNS may promote the survival of injured neurons of CN and RN, and may also inhibit the NOS expression of injured neurons of CN.

关 键 词：三七总皂甙 脊髓损伤 神经元存活 一氧化氮合酶 背核 红核 

分 类 号：R285.5[医药卫生—中药学]