低频脉冲磁场对大鼠皮质神经元损伤效应  

Damaging effects of low-frequency pulsed magnetic field on cortical neurons

作　　者：董娟[1] 李伯勤[1] 王旭平[1] 亓化振[1] 彭勃[2] 张莹[1] 

机构地区：[1]山东大学医学院超微结构室,济南250012 [2]山东大学控制与工程学院

出　　处：《中国公共卫生》2007年第5期592-594,共3页Chinese Journal of Public Health

基　　金：山东省自然科学基金(Y2001C28)

摘　　要：目的探讨低频脉冲磁场对大鼠皮质神经元的损伤效应及其可能机制。方法体外研究中,分离培养胎鼠大脑皮质神经元,对实验组神经元施加低频脉冲磁场辐射(频率15 Hz,平均场强0.1 mT),检测神经元的形态学变化、相对存活率和胞浆内游离Ca2+浓度的变化。体内研究中,对实验组大鼠施加与体外研究相同频率和强度的脉冲磁场辐射,检测脑组织形态学的变化。结果实验组神经元胞质内可见髓样体及线粒体聚集现象;实验组神经元相对存活率为81.17%,明显低于对照组(P<0.01);胞浆内游离Ca2+浓度比对照组升高17.29%(P<0.01)。实验组大鼠的皮质神经元出现异常改变。结论低频脉冲磁场辐射可导致培养的皮质神经元代谢异常、胞浆内游离Ca2+浓度升高及成年大鼠皮质神经元凋亡。Objective To investigate the damaging effects and the possible mechanism of low-frequency pulsed magnetic field using a combination of in vitro and in vivo approaches. Methods In vitro, primary cortical neurons were prepared from embryonic Wistar rats. The neurons of experimental group were exposed to Low-frequency Pulsed Magnetic Field（frequency： 15 Hz, average magnetic field intensity：0.1 mT） for 2 days, 3 times/d, 30 minutes/time. The morphology, the relative viability and the intracellular calcium level of neurons after exposure were determined. In vivo, the rats in experimental group were exposed to the same low-frequency pulsed magnetic field for 30 days, 45 min/d. The morphological changes were observed. Results In vitro, myeloid body and mitochondrial aggregation inside the cyton were observed in the experimental group, the relative viability of neurons in experimental group was 81.17 %, which decreased significantly than that of the control goup（ P 〈 0.01 ）, the intracellular calcium level of neurons in experimental group increased significantly （ P 〈 0.01 ）. In vivo, compared with the control group, abnormal changes of neurons were observed in experimental group. Conclusion The irradiating with low-frequency pulsed magnetic field resulted in metabolic abnormality, elevated intracellular free calcium level of neurons in vitro and apoptosis of neurons in vivo.

关 键 词：电磁场 神经元 大鼠 钙 

分 类 号：R122.4[医药卫生—环境卫生学]