B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的影响  

Effect of B-RAF-specific RNA interference on gastric cancer BGC823 cell line

作　　者：付浩[1] 赵丹懿[2] 孙秀菊[1] 滑君[1] 阎杨[1] 邱广蓉[1] 尚超[1] 富伟能[1] 孙开来[1] 

机构地区：[1]沈阳中国医科大学医学遗传教研室 [2]中国医科大学第一附属医院肿瘤外科,沈阳110001

出　　处：《遗传》2007年第5期537-540,共4页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目(编号:30571836)~~

摘　　要：为探讨B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的增殖和凋亡的影响,设计并合成B-RAF小分子干扰RNA(B-RAF-siRNA)和阴性对照siRNA,用TransMessenger介导转染胃癌BGC823细胞,RT-PCR分析检测胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达;MTT检测胃癌BGC823细胞增殖情况;流式细胞仪检测细胞凋亡情况,并与对照组进行比较。TransMessenger能够有效介导B-RAF-siRNA和阴性对照siRNA转染胃癌BGC823细胞,TransMessenger介导的B-RAF-siRNA有效地抑制胃癌BGC823细胞B-RAF以及Bcl-2基因的表达,与对照组相比,抑制率达90.0%以上,最高达100%;同时明显抑制胃癌BGC823细胞增殖;促进胃癌BGC823细胞的凋亡(P<0.01)。B-RAF基因特异的siRNA干扰能有效地抑制胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达,同时促进胃癌细胞凋亡和抑制胃癌细胞增殖。To investigate the influence of B-RAF-specific RNA interference on the proliferation and apoptosis of gastric cancer BGC823 cell line. B-RAF-siRNA and scramble-siRNA were synthesized and transfected into BGC823 cells by TransMessenger. The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry. Results showed that TransMessenger could effectively transfect B-RAF-siRNA and scramble-siRNA into BGC823 cells. B-RAF-siRNA significantly inhibited the expression of B-RAF gene and Bcl-2 gene in BGC823 cells by more than 90% to 100%. B-RAF-siRNA inhibited BGC823 cell proliferation and induced apoptosis （P 〈 0.01）. In conclusion, B-RAF-siRNA can effectively inhibit the expression of B-RAF gene and Bcl-2 gene, induce cell apoptosis and inhibit the proliferation of gastric cancer BGC823 cells.

关 键 词：RNA干扰 B-RAF基因 胃癌细胞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]