整合素αV、β3反义基因抑制裸鼠种植性肝癌血管生成的实验研究  

Antisense Intergrin αV and β3 suppresses the Angiogenesis of Implant Hepatocellular Carcinoma in Nude Mice

作　　者：贾欣永[1] 李杰[1] 徐宗珍[1] 韩旭[1] 张强波[1] 时昌文[1] 

机构地区：[1]山东省千佛山医院,山东济南250014

出　　处：《中国现代普通外科进展》2007年第2期153-155,共3页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金资助项目(2004ZX09)

摘　　要：目的:探讨整合素αV、β3反义基因对种植性肝癌血管生成的阻抑作用。方法:建立裸鼠肝癌模型,将反义基因表达载体αV/pcDNA3、β3/pcDNA3分别以αV、β3及αVβ3联合形式瘤内转染,同时设载体空壳组对照,检测整合素αV、β3蛋白及肿瘤组织微血管密度(MVD)的改变。结果:(1)实验组较对照组αV、β3蛋白表达显著降低(P<0.05);实验组之间差异无统计学意义(P>0.05)。(2)对照组、αV组、β3组、αVβ3组MVD分别为(17.53±1.88)、(16.06±1.92)、(15.83±2.00)、(14.86±1.69)条,αVβ3组与αV组、β3组及实验组与对照组之间MVD差异均有统计学意义(P<0.05)。结论:整合素αV、β3反义基因可明显下调整合素αV、β3蛋白表达,抑制肿瘤血管生成,联合应用效果更加显著。Objective： To investigate whether antisense integfin αV and β 3 gene has anti-angiogenesis activity in implanting hepatocellular arcinomas in nude mice. Methods： The antisense integrin αV and β 3 expression vectors were injected into HepG2 hepatomas established in nude mice, and tumorangiogenesis, integrin αV, integrin β 3, CD31 expressions were examined by immunostaining. Results： Gene transfer of antisenseintegrin αV and integrin β 3 expression vec- tors downregulated αV and β 3 protein expression inHepG2 tumors established in nude mice, and inhibited their vascularization. The median of microvessel density （MVD） in control, As-V, As- β 3 and As-αV β 3 group were 17.53,16.06,15.83 and 14.86 respectively, Antisense αV inhibited tumor angiogenesis more strongly than antisense 15 3; however antisense therapy that simultaneously targeted both integrin subunits was more effective than the respective monotherapies.Conclusions： Antisense gene therapy targeting αV and β3 integrins inhibited implantinghepatocellular arcinomas angiogenesis, which can be considered as an approach to treat hepatoceuular carcinomas.

关 键 词：整合素ΑV 整合素Β3 寡核苷酸类 反义 肝肿瘤 新生血管化 病理性 基因疗法 

分 类 号：R73-354[医药卫生—肿瘤] R735.7[医药卫生—临床医学]