脂多糖激活肝脏枯否细胞抗肿瘤作用的实验研究  

Effect of Lipopolysaccharide on Antitumor Activity of Mouse Kupffer Cells In Vitro

作　　者：周志伟[1,2] 万德森[1,2] 孙晓东[1,2] 王国强[1,2] 方淯靖[1,2] 潘志忠[1,2] 

机构地区：[1]华南肿瘤学国家重点实验室 [2]中山大学肿瘤防治中心腹科,广东广州510060

出　　处：《癌症》2007年第5期504-507,共4页Chinese Journal of Cancer

摘　　要：背景与目的:肝脏枯否细胞(Kupffer cells,KCs)是机体巨噬细胞中最大的群体,具有重要的免疫功能。本研究旨在探讨脂多糖(lipopolysaccharide,LPS)体外实验是否能增强肝脏枯否细胞的抗肿瘤活性。方法:酶消化法分离提取小鼠KCs,KCs与结肠癌细胞CT26混合培养,用WST-8法测定不同效靶比(KCs∶CT26)KCs对CT26细胞增殖的抑制情况;然后在同一效靶比(KCs∶CT26=10∶1)测定LPS不同浓度KCs对CT26细胞增殖的抑制情况。结果:随着KCs∶CT26的效靶比的增大,KCs对CT26细胞增殖的抑制率逐渐增高,效靶比为1∶1、2∶1、5∶1、10∶1、20∶1、50∶1、100∶1时,KCs对CT26细胞增殖的抑制率分别为7.86%、13.37%、15.51%、18.86%、18.79%、19.24%和19.98%。加入LPS后其抑制率明显增加,LPS浓度为0.001μg/ml、0.01μg/ml、0.1μg/ml、1μg/ml、10μg/ml、100μg/ml、1000μg/ml时,抑制率分别为16.86%、21.10%、25.64%、28.38%、26.78%、33.38%和38.70%。结论:LPS体外能够激活肝脏枯否细胞,从而增强其抗肿瘤活性。BACKGROUND ＆ OBJECTIVE： Kupffer cells （KCs） in the liver constitute the largest group of macrophages with strong immune functions. This study was to investigate the effect of lipopolysaccharide （LPS） on antitumor activity of mouse KCs in vitro. METHODS： KCs were isolated from the mouse liver by enzyme digestion, and co-cultured with mouse colon cancer cell line CT26 at different effector/target （KCs：CT26） ratios. The inhibitory effect of KCs on the proliferation of CT26 cells at different effector/target ratios, or at a ratio of 10：1 with treatment of LPS was measured by WST-8 colorimetric assay. RESULTS： The inhibition rate of CT26 celt proliferation was increased along with ascending effector/target ratios, the inhibition rates were 7.86%, 13.37%, 15.51%, 18.86%, 18.79%, 19.24%, and 19.98% when the effector/target ratios were 1：1, 2：1, 5：1, 10：1, 20：1, 50：1, and 100：1, respectively. At an effector/target ratio of 10：1, the inhibitory effect of KCs on CT26 cell proliferation was enhanced by LPS. the inhibition rates were 16.86%, 21.1%, 25.64%, 28.38%, 26.78%, 33.38%, and 38.7%.when treated with 0.001, 0.01, 0.1, 1, 10, 100, and 1 000 μg/ml LPS, respectively. CONCLUSION： LPS may activate KCs in vitro, and enhance its antitumor activity as well.

关 键 词：肿瘤 脂多糖 枯否细胞 抗肿瘤活性 

分 类 号：R73-3[医药卫生—肿瘤]