鼠切牙根端牙源性上皮细胞的体外培养及生物学特征  

In vitro culture and characterization of dental epithelial cells derived from the apical end of rat incisor

作　　者：江宏兵[1] 田卫东[2] 刘来奎[1] 徐艳[1] 

机构地区：[1]南京医科大学口腔医学院口腔医学研究所,210029 [2]成都四川大学华西口腔医学院

出　　处：《实用口腔医学杂志》2007年第2期165-168,共4页Journal of Practical Stomatology

基　　金：江苏省自然基金(编号:BK2006173)

摘　　要：目的:体外分离培养鼠切牙根端上皮细胞并观察其体外生物学特征。方法:体视显微解剖获取大鼠下颌切牙根端组织,组织块贴壁法培养分离牙源性上皮细胞,形态学结合免疫细胞化学观察其体外生物学特征。结果:牙源性上皮细胞自切牙唇侧颈环处迁出,呈圆形或方形的典型上皮细胞形态,Brdu标记显示其较强的体外增殖活力,上皮干细胞标记分子P63强阳性表达;随着传代,细胞趋向终末分化,碱性磷酸酶染色阳性。结论:鼠切牙唇侧颈环处可能存在牙源性上皮干细胞或前体细胞,为进一步研究牙源性上皮分化和牙组织工程提供细胞来源。Objective：To investigate the biological characteristics of dental epithelial cells derived from cervicalloop epithelium, in rat lower incisor. Methods： The apical end of the lower incisor was dissected from twn-day-old Sprague-Dawley rats by visual microseopy, and dental epithelial cells were obtained by euhurc of the apical end explants of the lower incisor. The biological characteristies of dental epithelial cells were determined by morphology and immunocytoehcmistry fluorescence. Results：Dental epithelial cells emigrated from the cervical loop of rat lower incisor, and epithelium-like morphologies with round-shape or square-shape was observed. Dental epithelial cells had a striking proliferation potential with a high level of BurdU labeling. As a marker of epithelium stem cells, P63 expression was observed in most early euhured cells. However, as cells were subcultured, dental epithelial cells,with the lower level of BurdU labeling,decreasing expression of P63 and increasing expression of alkaline phosphatase, were inclined to terminal differentiation. Conclusion：Stem cells or dental epithelial progenitor cells might reside in the cervical loop of the apical end of the rat lower incisor. The present study may provide an excellent cell source for study on the differentiation of dental epithelial cells and tooth regeneration.

关 键 词：牙源性上皮 细胞培养 分化 

分 类 号：R78[医药卫生—口腔医学]