牙支抗羊下颌骨牵张成骨的组织学观察  

Histological observation of mandibular advancement in goat using tooth-borne distraction osteogenesis device

作　　者：冯雪[1] 陈富林[1] 林珠[1] 段银钟[1] 

机构地区：[1]西安第四军医大学口腔医学院正畸科,710032

出　　处：《实用口腔医学杂志》2007年第2期180-182,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金资助(编号:30270373;30370374)

摘　　要：目的:用自制的牙支抗骨延长装置进行羊下颌骨延长的实验研究,观察其组织学特征。方法:将9只7～9月龄山羊,实验组分4组,每组2只,对照组1只,在下颌第2和第3前磨牙之间行骨皮质切开术后,将自制的颌骨延长装置黏固于牙齿之上,术后5d开始加力,每次0.25mm,2次/d。在开始加力后分别于8、16、32、48d时将动物处死,标本进行四环素荧光标记检查、HE染色及Mallory三色染色观察。结果:四环素荧光标记结果显示:在延长区域内有黄绿色荧光标记的新骨形成;HE染色及Mallory三色染色结果显示:新骨从牵引区两侧逐渐向中央延伸,融合后的骨小梁逐渐改建成熟,纤维束、新形成的骨小梁以及早期的各种细胞均表现为长轴与牵引力的方向一致。结论:新骨有沿着牵引力方向形成与改建的组织学特点。Objective：To observe the histological character of distraction osteogenesis （DO） in the mandible of goat using an intraoral tooth-supported distraction device. Methods：8 goats （7 -9 months of age） were divided ialo 4 groups with 2 goats per group, After a bilateral mandibular corticectomy between the second and third premolars, self-produced intraoral tooth-supported distraction devices were cemented, 5 clays after operation,the distraction devices were begun to be activated 0.25 mm eyery 12 hours to advance the anterior segment 8 mm in 16 days, The animals of 32 clays group were injected with tetracycline fluorescence labeing at 10 ml/kg once perweek from the beginning of DO. The animals were sacrificed on clay 8,16,32 and 48 separately post aetivation, and tetracycline fluorescence labeling,HE staining and Mallnry＇s 3-color staining were performed. Results：Tetracycline fluorescence labeling showed that there was green-yellow fluorescence labeling new bone within the distraction gap; HE and Mallory＇s trichrome staining showed that new bone gradually formed from bilateral sides, gradually blending and remodeling. The fiber bundles and newly formed trabeculas and various ceils in early phrases showed to he orientation-directed along the axis of distraction force. Conclusion： New bone can be formed and regenerated along the axis of distraction force within the distraction gap.

关 键 词：牵张成骨 牙支抗 

分 类 号：R782[医药卫生—口腔医学]