整合素αVβ3在实验性牙移动牙周组织骨改建中的表达  

Expression of integrin αVβ3 in bone remodeling of periodontium during experimental tooth movement

作　　者：孙秀梅[1] 孙新华[1] 

机构地区：[1]吉林大学口腔医学院正畸科,130041

出　　处：《实用口腔医学杂志》2007年第2期252-255,共4页Journal of Practical Stomatology

摘　　要：目的:检测正畸加力后牙周组织中整合素αVβ3的表达,探讨整合素αVβ3在实验性牙移动牙周组织骨改建中的作用。方法:35只大耳白兔随机分为7组,每组5只,分为对照组,实验1、3、5、7、14、21d组。于上颌第一磨牙与同侧切牙间置不锈钢螺簧,以0.78N力拉磨牙向近中。未加力组1周后处死动物,加力组于1、3、5、7、14、21d分批处死动物,制作组织学标本,通过原位杂交方法检测牙周组织中整合素αVβ3的表达。结果:实验各组均可见破骨细胞、成骨细胞、成骨细胞前体、成釉细胞、破牙质细胞和成牙本质细胞表达整合素αVβ3,但强度不一;骨细胞内未见表达。结论:整合素αVβ3参与了实验性牙移动牙周组织骨改建,参与了牙根的吸收和修复。Objective： To examine the expression of integrin αVβ3 in periodontium during experimental tooth movement, and then to propose the possible role of αVβ3 in bone remodeling during tooth movement. Methods： Thirty-five rabbits were divided into 7 groups （5 rabbits per group） ：untreated（ control）, 1,3, 5, 7, 14 and 21 d groups. A elastic： coil spring was ligated between the incisors and the first molar and exert a force of approximately 0.78 N to pull the first molar mesially. The animals were sacrificed at each time intervals（ 1,3,5,7,14 and 21 d） respectively and specimens were made to observe the expression of integrin αVβ3 in periodontium through in situ hybridlzation. Results：Different extent expression of integrin αVβ3 were seen in osteoclasts, osteoblast precursors, osteoblasts,odontoclasts and odontoblast except bone cells. Conclusions： lntegrin αVβ3 may play an important role in bone modeling, cementum resorption and its reparation during experimental tooth movement.

关 键 词：牙齿移动 骨改建 整合素ΑVΒ3 原位杂交 

分 类 号：R783.5[医药卫生—口腔医学]