塞来昔布对人子宫内膜腺癌细胞系HEC-1B侵袭力的影响  

Influence of celecoxib on the invasion of human endometrium adenocarcinoma cell line HEC-1B

作　　者：肖义涛[1] 罗来敏[1] 张睿[1] 

机构地区：[1]上海交通大学附属第六人民医院妇产科,上海200233

出　　处：《现代妇产科进展》2007年第4期274-277,F0003,共5页Progress in Obstetrics and Gynecology

基　　金：上海市科委资助项目(04JC14064);上海市卫生局重点科研项目(2005ZD006)

摘　　要：目的：探讨环氧化酶-2（COX-2）选择性抑制剂塞来昔布对人子宫内膜腺癌细胞系HEC-1B侵袭力的影响及其相关机制.方法：用噻唑蓝（MTT）法检测不同浓度塞来昔布作用HEC-1B细胞不同时间后细胞增殖的变化;不同浓度塞来昔布作用HEC-1B细胞24h后,Transwell小室法测定细胞的侵袭力,RT-PCR法测定细胞中COX-2 mRNA的表达.结果：MTT检测显示塞来昔布对HEC-1B细胞的增殖抑制作用呈时间依赖性和浓度依赖性;不同浓度塞来昔布作用HEC-1B细胞24h,Transwell侵袭实验结果表明,在50～200μmol/L塞来昔布组随着浓度的增加细胞穿膜数逐渐减少（P＜0.05）,RT-PCR结果显示,50～200μmol/L塞来昔布组随着药物浓度的增加COX-2 mRNA的表达逐渐减弱（P＜0.05）.结论：塞来昔布能抑制HEC-1B细胞增殖,降低细胞的侵袭力,其机制可能与下调细胞中COX-2 mRNA的表达相关.Objective ：To investigate the influence of cyclooxygenase-2 selective inhibitor celecoxib on the invasion of human endometrium adenocarcinoma HEC-1B cell line and the potential mechanism. Methods：The HEC-1B cell proliferation was examined by MTF assay. After being treated with different concentrations of celecoxib for 24hours, the cells invasion was detected by transwell chamber,the expression of COX-2 mRNA was analyzed by RT-PCR. Results：MTF results indicated that celecoxib could inhibit the proliferation of HEC-1B cells in a time- and dose-dependent manner. Treated with different concentrations of celecoxib for 24hours, transwell chamber assay indicated that the invasion of cells gradually decreased with the increase of celecoxib dose （50 - 200μmol/L group, P 〈 0. 05 ）. RT-PCR results indicated that the expression of COX-2 mRNA gradually decreased with the increase of celecoxib dose（ P 〈 0.05 ）. Conclusion：Celecoxib can inhibit the proliferation of HEC-1B cells and depress invasion of the cells. The mechanism may be associated with down-regulation of COX-2 mRNA expression in HEC-1B cells.

关 键 词：环氧化酶-2 塞来昔布 子宫内膜腺癌 增殖 侵袭 肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]