高效液相色谱-蒸发光散射检测器定量分析谷胱甘肽含量  

Quantification Analysis of Glutathione by HPLC with Evaporative Light Scatter Detector(ELSD)

作　　者：林志兴[1] 吕永琴[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院生物加工过程重点实验室

出　　处：《食品与发酵工业》2007年第4期119-121,共3页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(No.20325622;20576013;50373003);博士点基金资助项目(20030010004);国家十五攻关资助项目(2004BA411B05)

摘　　要：为了快速定量检测溶液中的谷胱甘肽(GSH)含量,建立了应用C18反相柱,HPLC-ELSD测定GSH含量的方法,确定了高效液相色谱分离条件。色谱条件为:色谱柱(Kromasil C18,250×4.6mm,5μm);流动相:V(H2O)∶V(CH3CN)∶V(TFA)=96.2∶3.7∶0.1;柱温:室温;流速:1.0mL/min;进样量:20μL;漂移管温度90℃;氮气流速:1.5L/min;i mpactor on模式;增益(Gain):1。结果表明,在此条件下,GSH浓度的自然对数与峰面积的自然对数成很好的线性关系(R2=0.9995);精密度2.15%;最低检测限30.73mg/L;加标回收率99.78%。该方法快速、简便、准确,适合从复杂体系中快速检测谷胱甘肽的含量。An HPLC-ELSD method for quantification analysis of glutathione was studied under the chromatographic conditions as mobile phase-a ratio of water, acetonitrile and TFA（96.2 ： 3. 7 ： 0.1） ; flow rate 1.0ml/min; column temperature-ambient temperature; flow rate -1.0mL/min. The detection conditions of ELSD were drift tube temperature -90℃, flow rate of N2-1.5L/min, impactor mode-on, and gain-1. The result shows that the detection limit is 30.73mg/L, and the standard deviation is 2. 15 %, and the average recovery is 99. 78%. This method is simple, rapid and accurate, and suitable to detect glutathione from complex system.

关 键 词：高效液相色谱 蒸发光散射检测器 定量分析 谷胱甘肽(GSH) 

分 类 号：TS207[轻工技术与工程—食品科学]