瘦素对大鼠肝纤维化的促进作用  

Enhancement of hepatic fibrosis by leptin in rat

作　　者：卢平[1] 高世蓉[1] 周力[2] 张永宏[2] 

机构地区：[1]华中科技大学同济医学院附属荆州医院消化科,湖北荆州434020 [2]贵阳医学院附属医院消化内科

出　　处：《解放军医学杂志》2007年第5期476-478,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究瘦素对实验性大鼠肝脏纤维化进程是否有促进作用。方法雄性Wistar大鼠126只,随机分成8组。正常对照组:不做处理;瘦素加四氯化碳Ⅰ～Ⅴ组:40%四氯化碳蓖麻油溶液皮下注射,同时给予不同剂量瘦素腹腔注射;四氯化碳造模组:40%四氯化碳蓖麻油溶液皮下注射;瘦素组:瘦素腹腔注射,生理盐水皮下注射。分别于第3、5周检测肝功能、肝纤维化指标及肝脏中瘦素表达的变化,光镜下观察组织学改变。结果瘦素各组肝功能、肝纤维化和肝脏中瘦素表达都增加,部分瘦素加四氯化碳组同四氯化碳组比较有明显差异(P<0.05),部分瘦素加四氯化碳组之间也有差异。瘦素组同对照组比较没有显著性差异。结论瘦素对大鼠肝纤维化的进程有促进作用,但不是一个独立的致肝纤维化因子。Objective To investigate the possibility of leptin on enhancing hepatic fibrosis induced by carbon tetrachloride in rat. Methods 126 male Wistar rats were divided into eight groups. The experimental hepatic fibrosis was induced by subcutaneous injection of carbon tetrachloride. Recombinant murine leptin was administered by intraperitoneal injection. Normal group.. Nothing was given to the rats. Leptin plus carbon tetrachloride groupI--V ： The rats were injected with 40% carbon tetrachloride together with leptin in different dosage by intraperitoneal injection everyday. Carbon tetrachloride group： The rats were injected with 40% carbon tetrachloride only. Leptin group： The rats were given leptin by intraperitoneal injection. The liver tissue was harvested 3 and 5 weeks after treatment for morphologic analysis with HE staining, and the extent of hepatic fibrosis was defined under light microscopy. Blood samples were collected to determine the serum transarninases （AST＆ AL T） and ALB. Serum procollagen ⅡЗ terminal peptide （PЗN） and hyaluronic acid （HA） were measured using radio-immunity technlque. The protein expressions of leptin in liver tissue were determined with immunohistochernistry assay. Results Compared with carbon tetrachloride group, significant differences were found in ALT, AST, ALB, PⅢN and HA levels in leptin plus carbon tetrachloride groups Ⅲ-Ⅴ, and significant differences in HA levels existed also between leptin plus carbon tetrachloride groups Ⅰ-Ⅱ and leptin plus carbon tetrachloride group Ⅴ. Compared with leptin plus carbon tetrachloride group Ⅰ and carbon tetmchloride group, the extent of hepatic fibrosis was enhanced in leptin plus carbon tetrachloride groupⅤ. No significant difference was found between normal group and leptin group. The protein expressions of leptin were significantly different compared among the leptin plus carbon tetrachloride group Ⅲ, leptin plus carbon tetrachloride group Ⅳ- and carbon tetrachloride group at the end of the fifth w

关 键 词：肝硬化 四氯化碳 瘦素 肝纤维化 促进作用 

分 类 号：R575.2[医药卫生—消化系统]