神经再生素对实验性高眼压兔视网膜i-NOSmRNA表达的影响  

Effect of nerve regeneration factor on expression of inducible-nitric oxide synthase in retina with experimental intraocular hypertension

作　　者：管怀进[1] 黄正如[1] 丁斐[2] 顾晓松[2] 

机构地区：[1]南通大学附属医院眼科,226001 [2]南通大学江苏省神经再生重点实验室,226001

出　　处：《眼科研究》2007年第5期325-328,共4页Chinese Ophthalmic Research

基　　金：江苏省自然科学基金(BK2001132);江苏省高校高新技术产业化项目基金(JH01-075)资助

摘　　要：目的探讨中药有效成分神经再生素(NRF)对实验性高眼压视网膜可诱导型一氧化氮合成酶(i-NOS)表达的影响。方法前房灌注法建立兔右眼实验性高眼压模型,分别给予NRF和磷酸盐缓冲液(PBS)。NRF组(n=6)在灌注前、灌注后4d、7d玻璃体腔内多点注射NRF4.5μg;PBS组(n=6)在灌注前、灌注后4d、7d玻璃体腔内注射等量0.1mol/LPBS;两组兔的左眼为正常对照组(n=12)。以逆转录聚合酶链反应(RT-PCR)法检测各组兔眼视网膜i-NOS基因表达量。结果NRF组兔视网膜i-NOSmRNA的表达量低于PBS组(P=0.000),但高于左眼正常对照组(P=0.000)。结论神经再生素能抑制青光眼视网膜i-NOSmRNA的表达,对青光眼视网膜神经节细胞可能具有保护作用。Objective It is generally believed nitric oxide is one of the toxins which result in retinal ganglion cells （RGCs） apoptosis. Nerve regeneration factor （NRF） from Chinese medicine has neuroprotective effect. This article studied whether NRF can protect RGCs with experimental intraocular hypertention by downregulating expression of inducible-nitric oxide synthase（i-NOS） in rabbit retina. Methods Experimental high intraocular pressure （lOP） models were established by balance salt solution perfusion into anterior chamber in the right eyes of 12 rabbits. 4.5 μg of NRF or 5 μl of PBS（0. 1 mol/L） was injected respectively into vitreous body cavity of right eyes of rabbits in NRF group（ n = 6） or PBS group （ n = 6） before perfusion and at 4,7 days in postperfusion. The left eyes of NRF group and PBS group served as normal control group, lOP was detected using Schitoetz tonometer before and after perfusion 4,7,14 days. The RT-PCR was used to examine the effect of NRF on expression of i-NOSmRNA in retina. Results No significant differences were found in lOP between NRF and PBS group at different time point （t = 0. 241,P = 0. 728 ; t = 0. 438, P = 0. 629 ; t = 0. 256, P = 0. 821 ; t = 1. 557, P = 0. 183 ）. The expression level of i-N OSmRN A in N RF group, PBS group and normal control group was （68.70 ± 14.55 ） , （ 146. 00 ± 5.01 ） and （ 50. 02 ± 3.50） , respectively. The expression level of i-NOSmRNA in NRF group （OD value） was significantly decreased compared with PBS group（ P = 0. 000）, but remained a higher level in comparison with normal control group. Conclusion NRF could down-regulate the expression of i-NOSmRNA in retina in high lOP eyes,which could protect retinal ganglion cells from glaucomatous neuropathy.

关 键 词：神经再生素 可诱导型一氧化氮合成酶 实验性高眼压 

分 类 号：R775[医药卫生—眼科]