急性高眼压后大鼠视网膜谷氨酰胺合成酶mRNA表达的变化  

Expression of glutamine synthetase mRNA in rat retina following acute intraocular hypertension

作　　者：张军富[1] 王竫华[1] 王大博[1] 白海青[1] 康菊[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003

出　　处：《眼科研究》2007年第5期373-375,共3页Chinese Ophthalmic Research

摘　　要：目的观察急性高眼压后大鼠视网膜谷氨酰胺合成酶(GS)mRNA的表达变化。方法前房灌注加压至110mmHg,维持60min。提取视网膜总RNA,以RT-PCR半定量GSmRNA。结果大鼠前房加压解除后4h,GSmRNA的表达较对照眼开始升高(P<0.05),至24h达高峰(P<0.01),36h降低(P<0.05),72h(P>0.05)恢复至正常水平。结论急性高眼压后大鼠视网膜GSmRNA的表达呈现明显升高过程,可能引起GS表达上调,促进谷氨酸转化为谷氨酰胺,从而减轻视网膜神经节细胞(RGCs)的损伤。Objective Retinal ischemia/reperfusion induce the necrosis and apoptosis of retinal ganglion cells（RGCs） , which release amount of glutathione and therefore aggravate the damage of RGCs. This study was curried out to observe the expression change of glutamine synthetase（GS） mRNA in the glaucomatous retina. Methods Acute intraocular hypertention was induced by increasing anterior chamber pressure to 110 mmHg for 60 rain in the left eyes of 30 Wistar rats using elevating the perfusion container to 150 cm,and anterior chamber incision alone was performed in other 5 rats, The right eyes of all the rats were as controls. Total RNA in retina was extracted and GSmRNA was semiquantitated by way of RT-PCR at 4,12,24,36,72 and 120 hours after removal of container, RNA purity was calculated at OD260 nm value. Results Expression level of GSmRNA was recorded as GSmRNA/GAPDHmRNA. In comparison with control group, the expression of GSmRNA was increased 4 hours after acute intraocular hypertention（ 1, 100 ±0. 122） ,peaked at 24 hours（ 1.511 ±0. 125） ,and descended at 36 hours（ 1. 282 ± 0. 131 ） ,showing a statistically significant difference in comparison with the control group （0. 852 ± 0. 115 ）, The expression of GSmRNA reverted to （0. 953 ± 0. 104） 72 hours after acute intraocular hypertention without significant difference compared with the control group. Conclusion The GSmRNA is overexpressed in acute hypertentive retina,which possibly results in the upregulation of GS that transforms glutamate into glutamine and ultimately mitigates the damage of retinal ganglion cells.

关 键 词：急性高眼压 视网膜 谷氨酰胺合成酶 

分 类 号：R775[医药卫生—眼科]