早期糖尿病小鼠血清和视网膜VEGF的表达及意义  

Expression and significance of serum and retinal VEGF in the early NOD mice

作　　者：李才锐[1] 姜德咏[1] 胥延[2] 郭小健[1] 洪卫[3] 李雯霖[1] 王平[1] 

机构地区：[1]中南大学湘雅二医院眼科,长沙博士410011 [2]昆明医学院第二医院眼科,410007 [3]大理医学院附属医院眼科,671000

出　　处：《眼科研究》2007年第5期376-378,共3页Chinese Ophthalmic Research

基　　金：湖南省科技厅科学研究项目资助(04SK3034/3)

摘　　要：目的探讨非肥胖性糖尿病小鼠血清和视网膜血管内皮生长因子(VEGF)的表达及二者之间的关系。方法非肥胖性糖尿病小鼠随机分为正常对照组(2、4、6、8、12周组,n=20)和糖尿病组(2、4、6、8、12周组,n=20),每组取4只小鼠摘除眼球取血并分离视网膜。ELISA方法检测血清及视网膜VEGF的表达。结果与正常对照组相比,糖尿病组血清及视网膜VEGF表达水平明显增高。结论糖尿病早期血清及视网膜VEGF表达增高,二者呈正相关,血清VEGF含量可作为判定糖尿病视网膜病变(DR)程度的参考指标。糖尿病早期视网膜VEGF的表达与多种因素有关。Objective Non-obese diabetes （NOD） mice can develop diabetes naturally. The diabetes incidences of female NOD mice is 85% but 10% in male mice. Vascular endothelium growth factor（VEGF） plays a key role in diabetic retinopathy. This study was designed to investigate whether serum and retinal VEGF levels were increased in the diabetic NOD mice and the relationship between them. Methods NOD mice were divided into normal control group（ non-diabetes mice） （2,4,6,8,12 weeks group, n = 20） and diabetes group （2,4,6,8,12 weeks group, n = 20） randomly. Four mice in each group were sacrificed and eyeballs were enucleated at schedule. Blood sample was obtained and retinas of experimental eyes were isolated. Retinal and serum VEGF were detected by ELISA （Enzyme-Linked hnmunosorbent Assay）. Results Compared with the normal control group,sermn and retinal VEGF levels were increased significantly in the diabetes group（ 12 weeks： 4.9 ± 0.4 pg/μg versus 0. 19 ±0. 1 pg/μg in serum sample,P 〈0.01 ;165 ±9 pg/μg versus 17 ±5 pg/μg in retinal sample,P 〈0.01 ）. A positive correlation was found between serum VEGF and retinal VEGF levels in the early diabetes（ 3, = 0. 990 2 ,P = 0. 001 ）. ConcIusion Serum VEGF levels can be used as a reference index to determine the seriousness of diabetic retinopathy. There are many factors associated with retinal VEGF expression in the early diabetes.

关 键 词：视网膜 血清 血管内皮生长凶子 非肥胖性糖尿病小鼠 

分 类 号：R774.1[医药卫生—眼科]