食管鳞状细胞癌组织中Survivin和Cox-2及PCNA表达临床意义的研究  

Clinical significance of Survivin,Cox-2 and PCNA expression in esophageal squamous cell carcinoma

作　　者：邹磊[1] 李道堂[1] 穆殿斌[2] 李辉[1] 罗景玉[1] 刘曙光[1] 

机构地区：[1]山东省肿瘤医院胸外科,山东济南250117 [2]山东省肿瘤医院病理科,山东济南250117

出　　处：《中华肿瘤防治杂志》2007年第7期534-537,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的探讨Survivin、Cox-2和PCNA表达与食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)临床病理因素及相互之间的关系。方法应用免疫组织化学(SP)方法检测Survivin、Cox-2蛋白和增殖细胞核抗原在51例食管鳞癌和30例切缘正常食管黏膜中的表达。结果Survivin和Cox-2在食管鳞癌的表达均高于切缘正常食管黏膜,P<0.01。Survivin蛋白在食管鳞癌组织中的表达阳性率与肿瘤分化程度相关,P<0.05。Cox-2蛋白的表达阳性率与食管鳞癌的临床病理特征无关,P>0.05。PCNA的表达阳性率与食管鳞癌的分化程度、淋巴结转移有关,差异有统计学意义,P<0.05。51例食管鳞癌组织中Survivin与Cox-2、PCNA之间具有显著正相关性,P<0.01;Survivin与PCNALI(反映细胞增殖活性的指标)具有正相关性,P<0.05;Cox-2与PCNA之间无相关性,P>0.05。结论Survivin与COX-2蛋白在食管鳞癌组织中均有较高的阳性表达,两者表达具有相关性,两者可能存在共同的激活机制,构成抑制食管鳞癌细胞凋亡的多种途径。Survivin表达与食管鳞癌的恶性进程有关。OBJECTIVE： This study was to investigate the correlation of Survivin,Cox-2 and PCNA expression to clinicopathologic factors in esophageal squamous cell carcinoma （ESCC）. METHODS： The presence and the level of expression of Survivin,Cox-2 and PCNA in 51 specimens from ESCC and 30 specimens from tissues along the incision margin（TIM）were detected by SP immunohistochemistry. RESULTS： There was very significant difference between the expression of Survivin and Cox-2 in ESCC and TIM, P〈0.01. The positive rates of Survivin was associated with differentiation grade of ESCC, P〈 0.05. The positive rates of Cox-2 was not assoeiated with tbe clinicopathologic features of ESCC, P〉0.05. The positive rates of PCNA was associated with differentiation grade and lymph node metastasis, P〈0.05. The expression of Survivin in ESCC was positively correlated with that of Cox-2,PCNA and PCNA LI, P〈0.01. The expression of Cox-2 was not correlated with that of PCNA, P〉0.05. CONCLUSIONS： There are high ex pression of Survivin and Cox-2 in ESCC with a positive corrclation between them. It is speculated that a common activation mechanism may coexists between the two genes,which construct multiple pathways contributing to the inhibition of apoptosis of ESCC. Survivin protein expression played an important role in the malignant progression of ESCC.

关 键 词：癌 鳞状细胞/代谢 食管肿瘤/病理学 淋巴转移 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]