人脂联素重组体体外对乳腺癌细胞侵袭和转移的抑制  

Inhibitory effect of recombinant human adiponection on invasion and metastasis of breast cancer cells in vitro

作　　者：潘兵[1] 路璐[1] 陈仲[1] 顾金龙[1] 赵明辉[1] 张春明[1] 韩佩珍[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所,天津300192

出　　处：《中国肿瘤生物治疗杂志》2007年第2期115-119,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.30500135)

摘　　要：目的:研究人脂联素重组体对人乳腺癌MDA-MB-231细胞体外侵袭能力的影响。方法:将MDA-MB-231细胞分设对照组和脂联素重组体加药(2.5、5.0、10、20、30μg/ml)组,药物作用一定时间后,分别以MTT法、Transwell小室实验,Chamber小室实验,黏附实验检测脂联素重组体对乳腺癌细胞增殖、侵袭、迁移和黏附能力的影响,以明胶酶谱法检测其对肿瘤细胞分泌MMP-2和MMP-9的影响。结果:脂联素重组体质量浓度高于5.0μg/ml时,对MDA-MB-231细胞生长具有明显的抑制作用(P<0.01);能明显地降低肿瘤细胞体外侵袭能力(P<0.01),侵袭抑制率随脂联素重组体质量浓度的升高而增加,介于22.64%～77.84%之间;脂联素重组体质量浓度高于2.5μg/ml时,明显地抑制肿瘤细胞的迁移能力(P<0.01);脂联素重组体对ECM和FN黏附能力影响的程度不同,对FN的敏感性大于ECM;脂联素重组体质量浓度大于2.5μg/ml时,明显抑制肿瘤细胞MMP-2和MMP-9的分泌(P<0.05或P<0.01),后者的分泌量随质量浓度的升高而下降。结论:脂联素重组体在大于2·5μg/ml时,可能通过保护基底膜不受破坏而抑制人乳腺癌细胞的侵袭能力。Objective： To investigate the effects of recombinant adiponectin （RA） on the invasive ability of human breast cancer cell line MDA-MB-231. Methods： MDA-MB-231 cells were divided into control group and RA（2.5, 5.0, 10, 20, and 30 μg/ml） treatment groups. The effect of RA on proliferation of MDA-MB-231 cells was measured by MrlT assay; the invasive and migration abilities of MDA-MB-231 cell were assayed in Transwell cell culture chamber; cell adhesion assay was carried out in a microcuhure well precoated with fibronectin. Gelatinolytic activities of both MMP-2 and MMP-9 secreted by cancer cells were measured by zymogrophy analysis. Results ： The growth and invasive ability of MDA- MB-231 cells were obviously inhibited when the concentration of RA was higher than 5.0 μg/ml （ P 〈 0.01 ） ; the inhibition of migration was in a concentration-dependent manner, with the inhibitory rate ranging from 22.64%-77.84%. When the concentration of RA was higher than 2.5 μg/ml, the migration of tumor and the secretion of MMP-2 and MMP-9 were obviously inhibited （P 〈0.05 ,P 〈0.01 ） ; the secretion of MMP-9 decreased with the increase of RA concentration. RA was more sensitive to FN and than to ECM. Conclusion： These results indicate that adiponectin is effective in suppressing tumor cell invasion when its concentration is 〉 2.5 μg/ml ; the mechanism might be the down-regulation of MMPs.

关 键 词：脂联素 乳腺癌细胞 侵袭 黏附 转移 

分 类 号：R730[医药卫生—肿瘤]