小麦抗白粉病基因Pm6的PCR标记鉴定  

Identification of PCR Markers Linked to Wheat Powdery Mildew Resistance Gene Pm6

作　　者：王瑞[1] 刘红彦[1] 李洪连[2] 王俊美[1] 伊艳杰[1] 

机构地区：[1]河南省农业科学院植物保护研究所,河南郑州450002 [2]河南农业大学植物保护学院,河南郑州450002

出　　处：《麦类作物学报》2007年第3期421-424,共4页Journal of Triticeae Crops

基　　金：河南省杰出青年科学基金项目(04120001400)

摘　　要：为了筛选出与小麦抗白粉基因Pm6连锁的PCR标记,选择位于小麦染色体2BL上的52个SSR和STS标记合成特异引物,对Pm6基因载体品种Timgalen和感病品种豫麦13及其F2代分离群体进行PCR分析,发现3个STS标记(Xwg996、KSUK948和XksuF37)和1个SSR标记(Xgwm47)与Pm6基因连锁,XksuF37、KSUK948、Pm6、Xwg996和Xgwm47五个位点之间的遗传距离分别为31.1、17.7、12.4和19.7cM。用标记Xwg996分析17个含其他抗白粉病基因的载体品种,结果表明,这个标记对Pm6基因有很强的专一性,可以应用于Pm6基因的分子鉴定和分子标记辅助育种。In orde to screen the PCR markers linked to wheat powdery mildew gene Pm6, 52 primer pairs based on the SSR（simple sequence repeat） and STS（sequence tagged site） markers on the long arm of chromosome 2B in wheat were synthesized to analyze the Pm6-carrying cultivar Timgalen, susceptible cultivar Yumai 13 and their F2 segregating population. The result showed that three STS markers Xwg996, KSUK948, XksuF37 and one SSR marker were linked to Pm6 gene. The order of the four markers and Pm6 gene locus was XksuFa7-KSUK948-Pm6-Xwg996-Xgwm47 with genetic distance of 31.1, 17.7, 12.4 and 19.7 cM for the four intervals, respectively. 17 Pm gene-carrying cultivars were tested with the marker Xwg996. It was found that the marker had better specialty and could be used in molecular identification and marker-assisted selecting （MAS） for Pm6 gene.

关 键 词：小麦 白粉病 Pm6基因 PCR标记 

分 类 号：S512.1[农业科学—作物学] S332.2