Effects of Andrographitis Paniculata Extracts on the Expression of CD40 in Endothelial Cells  

作　　者：张洁 李树生 万磊 

机构地区：[1]Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2007年第2期138-141,共4页华中科技大学学报（医学英德文版）

摘　　要：In order to investigate the expression of CD40 in endothelial cells （ECs） in a variety of injured conditions and the interventional role of Andrographitis Paniculata isolate （API0134）, the thoracic aorta ECs of guinea pigs were cultured in vitro until the third passage, incubated in the presence of media containing xanthine oxidase （XO） and xanthine （Xan） which produced oxygen free radical （OFR group）; oxidized-LDL （ox-LDL group）; XO, Xan and API0134 （OFR＋API0134 group）; or ox-LDL and API0134 （ox-LDL＋API0134 group）. The expression of CD40 in ECs was detected by immunofluorescence assay and reverse transcription-PCR （RT-PCR）. The results showed as compared with the control group, the expression of CD40 in ECs in OFR group and ox-LDL group was increased （P〈0.01）, but attenuated significantly in OFR＋ API0134 group and ox-LDL＋API0134 group （P〈0.05）. It was suggested that mPI0134 could protect atherosclerosis by inhibiting the expression of CD40 molecule in injured ECs.In order to investigate the expression of CD40 in endothelial cells （ECs） in a variety of injured conditions and the interventional role of Andrographitis Paniculata isolate （API0134）, the thoracic aorta ECs of guinea pigs were cultured in vitro until the third passage, incubated in the presence of media containing xanthine oxidase （XO） and xanthine （Xan） which produced oxygen free radical （OFR group）; oxidized-LDL （ox-LDL group）; XO, Xan and API0134 （OFR＋API0134 group）; or ox-LDL and API0134 （ox-LDL＋API0134 group）. The expression of CD40 in ECs was detected by immunofluorescence assay and reverse transcription-PCR （RT-PCR）. The results showed as compared with the control group, the expression of CD40 in ECs in OFR group and ox-LDL group was increased （P〈0.01）, but attenuated significantly in OFR＋ API0134 group and ox-LDL＋API0134 group （P〈0.05）. It was suggested that mPI0134 could protect atherosclerosis by inhibiting the expression of CD40 molecule in injured ECs.

关 键 词：API0134 endothelial cells CD40 ATHEROSCLEROSIS 

分 类 号：R285.5[医药卫生—中药学]