面向PCR微流控芯片的多通道温控系统  

Temperature-controlled System with Multicenter Based on PCR Biochip

作　　者：刘勋[1] 姚李英[1] 张印春[2] 陈涛[1] 左铁钏[1] 

机构地区：[1]北京工业大学激光工程研究院,北京100022 [2]北京工业大学电子信息与控制工程学院,北京100022

出　　处：《控制工程》2007年第3期340-342,共3页Control Engineering of China

基　　金：国家自然科学基金资助项目(50175002);北京市自然科学基金资助项目(3031001);国家自然科学基金重点资助项目(50335050)

摘　　要：基于PCR微流控芯片,研究和设计了一套多通道温度控制系统,实现了温控功能并应用于PCR扩增试验。系统由硬件电路搭建和控制软件编写共同实现。温度信号由AD590采集,经过CD4051选通,由TLC0832将模拟信号转换为数字信号,传送给AT89C2051。单片机根据负反馈算法对信号进行处理,得到控制指令,经过TLC5620得到模拟信号,控制驱动电路。利用汇编语言编写功能软件,包括A/D,D/A转换程序、跳转程序和系统闭环程序。通过自主设计的硬件电路和控制软件,实现了微型PCR微流控芯片的多通道温度控制系统。实验结果表明,系统符合PCR扩增原理的要求,升温迅速,控温准确,可用于PCR扩增试验。A temperature-controlled system with multiehannel of PCR biochips is designed for achieving the function of temperature control and applying in the PCR experiment. The hardware and the software actualize the system function.Temperature signals are collected by AD590, and then are selected by CD4051.The analog signal is transferred to the digital signal by TLC0832 and transmitted to AT-89C2051.Microcontroller treats the signals and implements temperature control, according to negative feedback algorithm.TiE5620 is used to change the control command to analog signal, and then controlle the drive part.The assembly language is used to write the A/D conversion, D/A conversion and closed loop program. The experimental results show that the system meets the requirements of PCR principle. It is accurate and the rise/fall rates of temperature are rapid.

关 键 词：PCR微流控芯片 温度控制 负反馈 单片机 

分 类 号：TP27[自动化与计算机技术—检测技术与自动化装置]