大肠癌中β-catenin和E-cadherin的表达及其临床意义  

Expression and clinical significance of beta-catenin and E-cadherin proteins in colorectal carcinoma

作　　者：戴文斌[1] 任占平[1] 陈蔚麟[1] 杜娟[1] 石喆[1] 唐德艳[1] 

机构地区：[1]广西柳州市人民医院广西医科大学第五附属医院病理科,广西柳州545001

出　　处：《右江民族医学院学报》2007年第3期340-342,共3页Journal of Youjiang Medical University for Nationalities

基　　金：广西柳州市科技攻关项目(2003自0217)

摘　　要：目的探讨β-catenin和E-cadherin在大肠癌发生、发展中的作用。方法应用免疫组织化学方法检测30例正常大肠粘膜、30例大肠腺瘤、10例大肠腺瘤恶变及50例大肠癌组织中β-catenin和E-cadherin蛋白的表达情况。结果大肠癌、大肠腺瘤恶变和大肠腺瘤β-catenin胞浆和(或)胞核异位表达率显著高于正常大肠粘膜(P均<0.01),大肠癌β-catenin异位表达率显著高于大肠腺瘤(P<0.01)。大肠癌中β-catenin、E-cadherin膜表达缺失率显著高于大肠腺瘤和正常大肠粘膜(P均<0.01),且与大肠癌组织分化程度、浸润深度、淋巴结转移、Dukes分期有关。结论β-catenin异位表达与大肠癌的发生有关,是大肠癌发生的早期事件;β-catenin、E-cadherin膜表达缺失与大肠癌的侵袭、转移有关。Objective To investigate the progression of oolorectal carcinoma. Methods roles of beta- catenin and E - cadherin play in the carcinogenesis and The expression and beta- catenin and E- cadherin proteins were detected by immunohistochemistry in 30 cases of normal eolorectal mueosa, 30 cases of eolorectal adenoma, 10 cases of malignant eolorectal adenoma and 50 cases of colorectal carcinoma tissues. Results The ectopic expression rates of beta- catenin and E- cadherin proteins in cell nucleus and cytoplasm of colorectal carcinoma, malignant colorectal adenoma and colorectal adenoma were all significantly higher than that of normal colorectal mucosa （ P 〈0.01）. The ectopic expression rate of beta- catenin in colorectal carcinoma was higher than the colorectal adenoma （ P 〈 0.01）. Compared to colorectal adenoma and normal mucosa, loss of membranous beta - catenin, E - cadherin expression rate in colorectal carcinoma were higher, there was statistically significant difference （ P 〈 0.01）, and the reduced membranous beta - catenin and E - cadherin expression were closely related with the differentiation degree, the invasive depth, the metastasis and Dukes stage in colorectal carcinoma tissues. cytoplasmic and nuclear beta - catenin expression may play a pivotal role in carcinogenesis and progression of colorectal carcinoma. Loss of mem- branous beta - catenin and E - cadherin expression may be related to the invasion and metastasis of colorectal carcinoma.

关 键 词：大肠 肿瘤 Β-连接素 E-钙粘素 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]