猪RYR_1基因的PCR-RFLP分析和氟烷基因利用的研究  

Studies on PCR RFLP Analysis of RYR 1 Gene and Utilization of Halothane Gene

作　　者：蒋思文[1] 熊远著[1] 邓昌彦 吴桢方 汪绍南 

机构地区：[1]华中农业大学畜牧兽医学院

出　　处：《中国畜牧杂志》1997年第2期9-10,21,共3页Chinese Journal of Animal Science

基　　金：农业部"八五"重点资助

摘　　要：采用ＰＣＲ－ＲＦＬＰ分析技术，以血液或猪毛提取ＤＮＡ为模板，建立了快速和简便的从分子水平上鉴别ＲＹＲ１基因型的方法，其准确率为１００％。不同品种（系）初步配套杂交结果显示：皮特兰×抗应激猪的胴体品质优于杜洛克×抗应激猪（Ｐ＜０．０５或Ｐ＜０．０１），而肉质优于皮特兰×应激群猪（Ｐ＜０．０１）。The PCR RFLP analysis technology was used to this study,the method of molecular genotyping pigs for RYR 1 gene has been builded for use of blood or hair root as source of DNA and template for PCR analysis. This method is rapid,simple and convenient,and its accurate is 100%. The crose test was carried out by using anti stress line and stress group as female parents and Duroc and Pietrain as male parents. The results showed that the carcass quality of Pietrian×anti stressis better than that of Duroc×anti stress(P<0.05 of P<0.01),while the meat quality is that of better than Pietrian×streess susceptible (P<0.01).

关 键 词：猪 RYR1基因 氟烷基因 胴体品质 肌肉品质 

分 类 号：S828.2[农业科学—畜牧学]