山萘黄素对高转移卵巢癌细胞HO-8910PM侵袭转移的影响  

Effect of Kaempferol on Invasion and Metastasis of Highly Metastatic Ovarian Carcinoma HO-8910PM Cells

作　　者：陈伟珠[1] 杨明[1] 刘新光[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,524023

出　　处：《实用癌症杂志》2007年第3期231-234,共4页The Practical Journal of Cancer

基　　金：广东省科技计划项目(NO.2002C30109);湛江科技局科技攻关计划项目(NO.020101)

摘　　要：目的研究山萘黄素(kaempferol)对人高转移卵巢癌细胞HO-8910PM转移相关能力的影响及其作用的机制。方法以台盼蓝拒染法检测山萘黄素对高转移卵巢癌细胞HO-8910PM细胞增殖的影响;采用Transwell小室法检测山萘黄素对HO-8910PM细胞的侵袭能力、趋化运动能力和黏附能力的影响。结果50μmol/L的山萘黄素作用细胞6h后能抑制HO-8910PM细胞体外侵袭人工基膜和趋化运动能力,抑制率分别为(10.53±2.71)%和(10.84±1.45)%,但与对照组相比,对黏附能力的影响无显著性差异。结论山萘菌素能抑制高转移卵巢癌细胞HO-8910PM的侵袭和运动能力,对黏附能力无显著影响。Objective To investigate the effect and its possible mechanism of kaempferol on the metastasis associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells. Methods Trypan blue stain assay was used to examine the effect of kaempferol on proliferation of HO-8910PM cells. Transwell Chamber assay was performed to determine the effect of kaempferol on invasion,migratory capacity and adhesion potential of HO-8910PM cells. Results Invasion and migration capacity of HO-8910PM cells were inhibited significantly after treatment with 50 pLmot/L kaempferol for 6h in vitro,and their inhibitory rates were（ 10.53 ± 2.71 ） % and （ 10.84 ±1.45 ） % respectively. Howerer,there was no significant difference on adhesion potential when compared with the control. Conclusion Kaempferol can inhibit the migration and invasion of HO-8910PM cells in vitro,but there was no obviously effect on adhesion

关 键 词：山萘黄素 侵袭 运动 转移 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]