戊四氮诱导单次惊厥幼鼠海马碱性成纤维细胞生长因子表达的研究  

Expression of basic fibroblast growth factor in the brain of epileptic rat induced with pentetrazole

作　　者：戴园园[1] 袁宝强[1] 魏海燕[1] 

机构地区：[1]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《徐州医学院学报》2007年第5期331-334,共4页Acta Academiae Medicinae Xuzhou

基　　金：徐州医学院附属医院课题(2006014)

摘　　要：目的利用戊四氮单次惊厥幼鼠模型,研究癫疒间单次惊厥发作后海马各区碱性成纤维细胞生长因子(bFGF)表达情况。方法建立戊四氮腹腔注射幼鼠单次惊厥发作模型,分成A组(癫疒间发作组)和B组(生理盐水对照组)。每个组后按造模后1天、7天、14天分为3组。HE染色观察病理形态改变,免疫组化观察海马bFGF表达变化。结果病理形态上A组可见海马神经元变性、坏死,胶质细胞增生,B组形态学无明显变化。bFGF表达:A组发作后1天bFGF表达增高最明显(P<0.01),以CA1区和齿状回明显,以后逐渐下降,7天时仍高于B组(P<0.05),14天时下降到较低水平,和B组无显著性差异(P>0.05)。B组各时间点bFGF表达无显著性差异。结论幼鼠单次惊厥发作后海马bFGF表达增加,在发作后7天内较为明显。bFGF在癫疒间后脑损伤的自我修复中可能发挥着重要的作用。Objective To study the expression of basic fibroblast growth factor （bFGF） in the hippocampus of epileptic pedo - rats at different time points and investigate its bearing on epilepsy. Methods Forty - two 14 - day old SD pedo - rats were randomly divided into two groups ： A （ epilepsy model group） and B （ normal saline group）. Pentetrazole was used to induce seizure models. The expression of bFGF in the hippocampus of rats was studied by immunofluorescent labeling technique. H - E staining was done for pathomorphological study. Results In group A, neuronal degeneration and necrosis in the hippocampus, and cellular proliferation of glial cells as well, were noticed; the expression of bFGF was increased, most marked 1 day after seizure （ P 〈 0.01 ）, continued to be higher for 7 days （ P 〈 0.05 ）, and then declined and approached the control level 14 days later （P 〉0.05）. Conclusion The expression of bFGF in hippoeampus of 14 - day rat is increased after acute epilepsy, significantly higher for 7 days after seizure, bFGF may play an important role in the self - plerosis after brain injury induced by epilepsy in pedo - rats.

关 键 词：癫痫 碱性成纤维细胞 生长因子 海马 戊四氮 

分 类 号：R742.1[医药卫生—神经病学与精神病学]