色甘酸钠对大鼠肠缺血/再灌注左心功能变化的影响  

Cromolyn sodium ameliorates rat left cardiac function during intestinal ischemia-reperfusion

作　　者：罗刚健[1] 甘小亮[1] 沈宁[1] 黑子清[1] 黎尚荣[1] 陈立新[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广东广州510630

出　　处：《南方医科大学学报》2007年第5期650-653,共4页Journal of Southern Medical University

基　　金：广东省中医药管理局基金(1040051)~~

摘　　要：目的探讨肠缺血/再灌注对大鼠心功能及心肌病理损伤的影响及色甘酸钠的保护作用。方法SD大鼠32只,随机分为四组(n=8):假手术组(A组)、模型组(B组)、色甘酸钠I组(C组,模型+色甘酸钠50mg/kg)及色甘酸钠II组(D组,模型+色甘酸钠25mg/kg)。复制肠缺血-再灌注损伤模型,C、D组在再灌注前15min分别腹腔给予色甘酸钠50、25mg/kg。分别于缺血前即刻(基础值T0)、缺血15(T1)、30(T2)、45(T3)min、再灌注3(T4)、5(T5)、10(T6)、15(T7)、45(T8)、60(T9)min时记录心率(HR)、左室收缩压峰值(LVSP)及左室内压力变化最大速率(+dp/dtmax、-dp/dtmax)。在实验结束断头处死动物,光镜下观察心肌组织学改变。测定心肌组织匀浆中MDA含量和SOD活性。结果(1)与基础值T0比较,再灌注T8、T9时的HR、+dp/dtmax、-dp/dtmax及LVSP均降低(P<0.05)。(2)与A组比较,再灌注45和60min时,B、C和D三组HR、+dp/dtmax、-dp/dtmax及LVSP均降低(P<0.05)。C和D组HR、+dp/dtmax、-dp/dtmax及LVSP高于B组(P<0.05)。(3)心肌损伤病理评分显示B、C及D组较A组明显升高,C组病理评分低于B组。(4)B组MDA含量明显高于A组(P<0.05),C组MDA含量明显低于B组(P<0.05),B、D组SOD活性明显低于A组(P<0.05),C组SOD活性明显高于B组(P<0.05)。结论色甘酸钠能一定程度减轻肠缺血/再灌注心肌损伤,改善心功能。Objective To investigate cardiac function impairment and myocardial injury in rats with intestinal ischemia-reperfusion and the protective effect of cromolyn sodium. Methods Thirty-two SD rats were randomized into 4 groups （n=8）, namely the sham operation group, model group, 50 mg/kg cromolyn sodium group, and 25 mg/kg cromolyn sodium group. Intestinal damage was induced by clamping the superior mesenteric artery for 45 min followed by reperfusion for 60 min. Cromolyn Sodium was administrated intaperitoneally 15 min before reperfusion. The heart rate （HR）, left ventricle pressure （LVSP）, and the maximal/minimum rate of LVSP （＋dp/dtmax -dp/dtmax） were sacrificed immediately before ischemia （baseline, To）, at 15 min （T1）, 30 rain （T2）, 45 min （T3） ofischemia, and at 3 min （T4）, 5 min （T5） 10 min （T6）, 15 rain （T7）, 45 min （T8）, 60 min （Tg） of reperfusion. At the end of the experiment, the rats were executed and the hearts were immediately removed for observation of the pathological changes and determination of MDA contents and SOD activity. Results Compared with the baseline T07, the HR, ＋dp/dtmax -dp/dtmax and the LVSP were decreased significantly at T8 and T9 in the model group and the two cromolyn sodium groups （P〈0.05）. Compared with the sham operation group, these indices were also significantly decreased at T8 and T9 in the model group and the two cromolyn sodium groups, but the model group had significantly lower levels for these indices at T8 and T9 than the two cromolyn sodium groups （P〈0.05）. The score of myocardial injury in the model group and the two cromolyn sodium groups were significantly higher than that of group A, and 50 mg/kg cromolyn sodium group had lower score than the model group （P〈0.05）. The rats in the model group had significantly higher MDA levels than those in the sham operation group and the 50 mg/kg cromolyn sodium group. SOD activities in the model group and 25 mg/kg cromolyn sodium group was lower than th

关 键 词：缺血 肠 再灌注 心功能 色甘酸钠 

分 类 号：R363[医药卫生—病理学]