能转化鹅去氧胆酸为熊去氧胆酸的三株厌氧梭菌的鉴定  

IDENTIFICATION OF THREE CLOSTRIDIA STRAINS CAPABLE OF BIOTRANSFORMING CHENODEOXYCHOLIC ACID TO URSODEOXYCHOLIC ACID

作　　者：黄爱珉 周德庆[2] 胡宝龙[2] 

机构地区：[1]暨南大学生物学系 [2]复旦大学微生物学与微生物工程系

出　　处：《复旦学报（自然科学版）》1990年第2期180-188,共9页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金

摘　　要：对三株能转化鹅去氧胆酸(chenodeoxycholic acid,CDCA)为熊去氧胆酸(ursodeoxycholic acid,UDCA)的厌氧梭菌进行了系统的鉴定。通过电镜、API-20A数值鉴定系统、气相色谱分析和(G+C)mol%等测定表明,HS-10为A型产气荚膜梭菌(Clostridium perfringens),DL-20和LQ-29同属丁酸梭菌(Clostridium butyricum)。这三个菌株均具有独立转化CDCA为UDCA的能力。Three strains of clostridia which biotransform chenodeoxycholic acid (ODCA)to ursodeoxycholic acid(UDOA) were isolated from 640 soil samples by the 'syrin-ge method' designed by us. The three strains were identified as Clostridium per-fringens type A (strain HS-10) and C. butyricum (strains DL-20 and LQ-29) byover 40 items, such as electron microscopy, API-20A numerical taxonomy system,GLC, (G+C)mol%, and antitoxin neutralization and so on. It has not been repor-ted before that these two species of clostridia (three strains) are capable of bioco-verting CDCA to UDCA individually.

关 键 词：熊去氧胆酸 鹅去氧胆酸 厌氧梭菌 

分 类 号：TQ464.5[化学工程—制药化工]