不同程度急性等容性血液稀释联合控制性降压对大鼠血清S100B蛋白和脑组织形态学的影响  

Effects of different degrees of acute normovolemic hemodilution combined with controlled hypotension on serum S100B and cerebral morphology

作　　者：葛亚丽[1] 曾因明[1] 宋娟[1] 段满林[2] 周志强[2] 

机构地区：[1]江苏省麻醉医学研究所、江苏省麻醉学重点实验室,徐州221002 [2]南京军区南京总医院麻醉科

出　　处：《国际麻醉学与复苏杂志》2007年第2期105-107,110,共4页International Journal of Anesthesiology and Resuscitation

摘　　要：目的观察四种程度急性等容性血液稀释联合控制性降压对大鼠血清S100B浓度及脑组织形态学的影响。方法40只雄性SD大鼠（300g±25g）随机分为假手术组和四种程度血液稀释联合控制性降压组（Hct分别为30％、25％、20％、15％），每组8只。应用硝普钠控制MAP50mmHg～60mmHg，持续1h。血液稀释后3h处死大鼠，应用ELISA法测定血清S100B的浓度，应用电镜观察脑海马CA1区神经元超微结构。结果Hct20％、Hct15％实验组大鼠血清S100B浓度较假手术组明显上升；Hct20％、Hct15％实验组大鼠海马CA1区神经元线粒体评分明显高于假手术组，并可见染色质聚集，核膜皱褶及神经元核周胶质细胞聚集现象。结论重度急性等容性血液稀释（Hct≤20％）联合控制性降压后大鼠脑海马CA1区线粒体严重变性，并可见明显的胶质细胞反应，导致血清S100B浓度的明显上升。Objective To explore the effects of different degrees of acute normovolemic hemodilution combined with controlled hypotension on serum S100B concentration and cerebral morphology. Methods Forty adult Male Sprague-Dawley rats weighing 300 g ± 25 g were randomly assigned to 5 groups：sham-operation group, and 4 treated groups（ hemodiluton and controlled hypotension, with Hct of 30%, 25%, 20%, 15%, respectively）, 8 animals in each group. 30 min after hemodilution, she animals received sodium nitroprusside-induced hypotension （ MAP 50 mm Hg-60 mm Hg） for 1 h, then the rats were euthanatized 3 h after hemodiluton. The concentration of serum S100B was assessed by ELISA at baseline and the end of experiment. The uhrastructure changes of cerebral cells in the CA1 of rat hippocampus were analyzed with electron microscopy. Results At the end of the experiment, the serum S100B concentration was much higher in Hct 20% and Hct 15% groups than that in the sham-operation group. In the CA1 of hippocampus, the scores of mitochondria were higher in Hct 20% and Hct 15% groups, chromatin condensation and karyotheca crimple could be seen in these two groups, as well as the phenomena of swollen glia assembling around the neurons. Conclusion Obvious glial activation and degeneration of mitochondria in the CA1 of rat hippocampus occurred after severe acute normovolemic hemodilution （ Hct ≤ 20% ） combined with controlled hypotension,which induced increased concentration of serum S100B.

关 键 词：急性等容性血液稀释 控制性降压 S100B 超微结构 胶质细胞 

分 类 号：R[医药卫生]